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Computer image analysis to quantify and analyze stable transformation identified using the histochemical GUS assay

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Abstract

A precise and efficient protocol using computer image analysis for quantifying the area of explants that show stable expression of the GUS reporter gene is described. This protocol offers the advantages of quantifying results of the histochemical GUS assay thereby allowing statistical analysis of results, while still retaining information on tissue or organ-specific activity.

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Abbreviations

IOD:

integrated optical density

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Authors and Affiliations

  1. Department of Horticulture, Lincoln University, PO Box 84, Canterbury, New Zealand

    Catherine Owens y de Novoa

  2. New Zealand Crop & Food Research Ltd, Private Bag 4704, Christchurch, New Zealand

    Graeme Coles

Authors
  1. Catherine Owens y de Novoa
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  2. Graeme Coles
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Owens y de Novoa, C., Coles, G. Computer image analysis to quantify and analyze stable transformation identified using the histochemical GUS assay. Plant Mol Biol Rep 12, 146–151 (1994). https://doi.org/10.1007/BF02668375

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  • DOI: https://doi.org/10.1007/BF02668375

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