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A new model for the tertiary structure of 5S ribonucleic acid in plants

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Abstract

Chemical, enzymatic and physicochemical methods of a structural analysis of 5S rRNAs in lupine, wheat germ, and other plants led us to propose a new three-dimensional model of these molecules The main features of the model are tertiary interactions between the β- and γ-domains of the molecule, specifically nucleotides (34)CCCA(37) in loop C and nucleotides (85)GGGU(88) in loop D. In addition we propose tertiary base-pairing in A100-U53 between loops B and E. We have confirmed this model by NMR spectroscopy and by chemical modification with diethylpyrocarbonate. Our results are consistent with the proposed model and are also applicable to all eukaryotic 5S rRNAs. Our model is clearly differentiated from others by intramolecular tertiary hydrogen bonds between the two domains.

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Abbreviations

DSC:

differential scanning calorimetry

EF-1, EF-2:

polypeptide elongation factors 1 or 2

Mu-MoLV:

murine Moloney leukemia virus

NOE:

nuclear Overhauser effect

TAR:

tat-responsible element of human immunodeficiency virus

TFIIIA:

transcription factor type IIIA fromXenopus

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Authors and Affiliations

  1. Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Noskowskiego 12, 61-704, Poznan, Poland

    Miroslawa Z. Barciszewska & Jan Barciszewski

  2. Institut für Biochemie, Freie Universität Berlin, Thielallee 63, Berlin 33, Germany

    Volker A. Erdmann

Authors
  1. Miroslawa Z. Barciszewska
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  2. Volker A. Erdmann
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  3. Jan Barciszewski
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Barciszewska, M.Z., Erdmann, V.A. & Barciszewski, J. A new model for the tertiary structure of 5S ribonucleic acid in plants. Plant Mol Biol Rep 12, 116–131 (1994). https://doi.org/10.1007/BF02668373

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