Abstract
Erythro-sphingosine was obtained from sphingomyelin by alkaline hydrolysis. N-p-nitrobenzoyl-sphingosine, N-p-aminobenzoyl-dihydrosphingosine and dihydrosphingosine-protein were synthesized.
It was found that dihydrosphingosine-protein can produce a specific antibody which can be detected by the complement fixation test and by Ouchterlony's double diffusion method in agar. The determining factor of dihydrosphingosine may be due to the hydroxy groups at C1 and C3.
In the course of experimental allergic encephalomyelitis, the cross-reactivity of rabbit antisera against spinal cord, and with psychosine-protein in particular, was observed by the complement fixation test and by the Arthus reaction.
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Abbreviations
- GVB:
-
gelatin-veronal buffer containing 1.5×10−4 M CaCl2 and 5×10−4 M MgCl2, pH 7.5
- SE:
-
dihydrosphingosine—egg albumin
- SS:
-
dihydrosphingosine—Serum albumin
- CE:
-
ceramide—egg albumin
- PS:
-
psychosine—serum albumin
- PE:
-
psychosine—egg albumin
- GE:
-
glucose—egg albumin
- LS:
-
lactose—serum albumin
- LE:
-
lactose—egg albumin
- Gal-NS:
-
N-acetyl galactosamine—serum albumin
- GNE:
-
N-acetyl glucosamine—egg albumin. C1H50 refers to the hemolytic unit of complement, i.e., that amount required to lyse 50% of 5.0×108 sensitized red cells under standard conditions (24)
References
Yamakawa, T., R. Irie and M. Iwanaga, J. Biochem.48, 490 (1960).
Makita, A., and T. Yamakawa, Ibid.51, 124 (1962).
Yamakawa, T., A. Makita, T. Taketomi, S. Handa, and S. Yokoyama, Abstracts, 6th International Congress of Biochemistry, New York, 1964.
Handa, S., Japan J. Expr. Med.33, 347 (1963).
Taketomi, T., and T. Yamakawa, J. Biochem.54, 444 (1963).
Carter, H. E., O. Nalbandov and P. A. Tavormina, J. Biol. Chem.192, 197 (1951).
Carter, H. E., D. Shapiro and J. B. Harrison, J. Am. Chem. Soc.75, 1007 (1953).
Joffe, S., M. M. Rapport and L. Graf, Nature197, 60 (1963).
Niedieck, B., and E. Kuwert, Z. Immun. Allerg.125, 470 (1963).
Brady, R. O., and E. D. Trams, Ann. Rev. Biochem.33, 75 (1964).
Babers, F. H., and W. F. Goebel, J. Biol. Chem.105, 473 (1934).
Westphal, O., and H. Schmidt, Anal. Chem.575, 84 (1952).
Kabat, E. A., and M. M. Mayer, “Experimental Immunochemistry,” Charles C Thomas, Publisher, Springfield, Ill., 1961, p. 209.
Goldstein, G., I. S. Slizys and M. M. Chase, J. Exper. Med.114, 89 (1961).
Fujino, Y., and H. E. Carter in “Biochemical Problems of Lipids,” Popjak, G., and E. Breton, (ed.), Butterworths Scientific Publications, London, 1956, p. 115.
Yamakawa, T., in “Biochemistry and Medicine of Mucopolysaccharides,” F. Egami and Y. Oshima, Maruzen Company Ltd., Tokyo, 1962, p. 136.
Waksman, B. H., H. Porter, M. B. Lees, R. D. Adams and J. Folch, J. Exp. Med.100, 451 (1954).
Kies, M. M., and E. C. Alvord, Jr., in “Allergic Encephalomyelitis,” M. M. Kies, and E. C. Alvord, Jr., (Eds.), Charles C Thomas, Publisher, Springfield, Ill., 1959, p. 293.
Robertson, D. M., R. Blight, and C. E. Lumsden, Nature196, 1005, (1962).
Olitsky, P. K., and J. M. Lee, J. Immunol.71, 419 (1953).
Lumsden, C. E., E. A. Kabat, A. Wolf and A. E. Bezer, J. Exp. Med.92, 253 (1950).
Good, R. A., in “Mechanisms of Demyelination,” A. S. Rose, and C. M. Pearson, (Eds.), McGraw-Hill Book Company Ltd., N.Y., 1963, p. 119.
Waksman, B. H., Ibid. in “ p. 170.
Kabat, E. A., and M. M. Mayer, “Experimental Immunochemistry,” 2nd ed., Charles C Thomas, Publisher, Springfield, Ill., 1961, p. 149.
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Taketomi, T., Yamakawa, T. Antigenic properties of a synthetic protein complex with glycolipids and related substances. Lipids 1, 31–40 (1966). https://doi.org/10.1007/BF02668122
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DOI: https://doi.org/10.1007/BF02668122