Abstract
A simple and quick method for the quantitative determination of aldehydes in oxidized oils was established. The analysis was based on the reaction of N,N-dimethyl-p-phenylenediamine (DPPD) with aldehydes in the presence of acetic acid. Reaction products were determined by visible absorption at wavelengths of 400, 460 and 500 nm. The errors of the DPPD method in the analyses of total aldehydes in authentic mixtures of various aldehydes were less than 5%, and this method could be used successfully to determine aldehyde contents in oxidized vegetable and fish oils. The reaction time of the DPPD method (10 min at 30°C) is much shorter than that of the conventional method that uses 2,4-dinitrophenylhydrazine as a reagent (30 min at 60°C). The simplicity of the procedure enables a quick determination of aldehyde contents in oxidized oil samples.
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References
Frankel, E.N.,Prog. Lipid Res. 22:1 (1982).
Frankel, E.N., inFlavor Chemistry of Fats and Oils, edited by D.B. Min and T.H. Smouse, American Oil Chemists’ Society, Champaign, IL, 1985, p. 1.
Schauenstein, E., H. Esterbauer and H. Zollner, inAldehydes in Biological Systems. Their Natural Occurrence and Biological Activities, Pion Publishing, London, 1977, p. 1.
Esterbauer, H., inFree Radicals in Liver Injury, edited by G. Poli, K.H. Cheeseman, M.U. Dianzani and T.F. Slater, IRL Press, Oxford, 1986, p. 29.
Henick, A.S., M.F. Benca and J.H. Mitchell, Jr.,J. Am. Oil Chem. Soc. 31:88 (1954).
List, G.R., C.D. Evans, W.K. Kwolek, K. Warner, B.K. Boundy and J.C. Cowan,Ibid. 51:17 (1974).
White, P.J., and E.G. Hammond,Ibid. 60:1769 (1983).
Przybylski, R., and F.W. Hougen,Ibid. 66:1465 (1989).
Takagi, T., and K. Miyashita,Ibid. 64:407 (1987).
Takagi, T., Y. Mitsuno and M. Masumura,Lipids 13:147 (1978).
Official Method Cd 8-53,Official and Tentative Methods of the American Oil Chemists' Society, Vols. I & II, 3rd edn., American Oil Chemists’ Society, Champaign, IL, 1973.
Mizuno, G.R., and J.R. Chipault,J. Am. Oil Chem. Soc. 42:839 (1965).
Chiba, T., M. Takazawa and K. Fujimoto,Ibid. 66:1588 (1989).
Dugan, P.R.,Analytical Chemistry 33:696 (1961).
Dugan, P.R., and R.D. O’Neill,Ibid. 35:414 (1963).
Day Jr. R.A., and A.L. Underwood, inQuantitative Analysis, 1st edn., Prentice-Hall, Inc., Englewood Cliffs, N.J., 1958, p. 386.
Jones, L.A., J.C. Hohnes and R.B. Seligman,Anal. Chem.28:191 (1956).
Frankel, E.N.,Chem. Phys. Lipids 44:73 (1987).
Miyashita, K., N. Hara, K. Fujimoto and T. Kaneda,Agric. Biol. Chem. 49:2633 (1985).
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Miyashita, K., Kanda, K. & Takagi, T. A simple and quick determination of aldehydes in autoxidized vegetable and fish oils. J Am Oil Chem Soc 68, 748–751 (1991). https://doi.org/10.1007/BF02662165
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DOI: https://doi.org/10.1007/BF02662165