Abstract
A new, two-dimensional thin-layer chromatographic system was established to provide good separation of the unsaponifiable fractions from the seed oils of three wildSesamum species, [S. alatum, Thonn.;S. radiatum, Schum and Thonn.; andS. angustifolium, (Oliv.) Engl.] and of the cultivatedS. indicum, L. The system utilizes silica gel plates and n-hexane/diethyl ether (7:3, v/v) and chloroform/diethyl ether (9:1, v/v) as mobile phases in the first and second directions, respectively. The system could be used for qualitative studies and as a preparative technique for subsequent quantitative gas chromatographic separations in chemotaxonomic and related studies onSesamum spp.
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Kamal-Eldin, A., Yousif, G. & Appelqvist, L.Å. Thin-layer chromatographic separations of seed oil unsaponifiables from fourSesamum species. J Am Oil Chem Soc 68, 844–847 (1991). https://doi.org/10.1007/BF02660599
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DOI: https://doi.org/10.1007/BF02660599