Immunogenetics

, Volume 44, Issue 2, pp 134–142 | Cite as

Sequence and characterization of twoHSP70 genes in the colonial protochordateBotryllus schlosseri

  • Melinda B. Fagan
  • Irving L. Weissman
Original Paper

Abstract

Two genes belonging to the heat shock protein 70 gene family have been cloned from the colonial protochordateBotryllus schlosseri. The two intronless genes(HSP70.1 andHSP70.2) exhibit 93.6% sequence identity within the predicted coding region, and 83.3% and 81.7% sequence identity in the 5′ and 3′ flanking regions, respectively. The predicted amino acid sequences are 95% identical and contain several signatures characteristic of cytoplasmic eukaryoticHSP70 genes (Gupta et al. 1994; Rensing and Maier 1994). Northern blotting and sequence analysis suggest that both genes are heat-inducible merebees of theHSP70 gene family. Given these characteristics,HSP70.1 andHSP70.2 appear to be good candidates for protochordate homologues of the major histocompatibility complex-linkedHSP70 genes of human, mouse, and rat (Milner and Campbell 1990; Walter et al. 1994). Further experiments to determine whether there is functional evidence for such similarity are in progress.

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Melinda B. Fagan
    • 1
  • Irving L. Weissman
    • 1
  1. 1.Department of Pathology, Stanford University Medical CenterStanford UniversityStanfordUSA

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