Abstract
The coagulation variables thrombin time (TT), activated partial thrombin time (APTT) and prothrombin time (PT), were investigated in mouse plasma. TT and APTT clotting times were determined using a KC10 coagulation analyser and test kits Dia Thrombin (bovine thrombin diluted to a concentration of 2.5 U/l) and Dia Celin-L (rabbit brain cephaloplastin, activated with complexed Kaolin), respectively. PT was determined with IL Test™ Hepatocomplex (rabbit brain thromboplastin and calcium ions) using an ACL 200. Furthermore, the test procedures were also used to assess the anticoagulant status of mice treated orally with Melagatran, a thrombin inhibitor. The results showed that the kits could be used successfully on mouse plasma to measure the effect of a thrombin inhibitor on haemostasis.
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References
Bernardi C, Moneta D, Brughera M et al. (1996) Haematology and clinical chemistry in rats: Comparison of different blood collection sites. Comp Haematol Int 6:160–166
Dameron GW, Weingand KW, Duderstadt JM et al. (1992) Effect of bleeding site on blood coagulation results on clinical laboratory testing of rats: orbital venous plexus versus posterior vena cava. Lab Anim Sci 42:299–301
Edwards CJ, Fuller J (1993) Effect of bleeding site on coagulation results in the Charles River outbred albino SD rat. Comp Haematol Int 3:40–42
Gustafsson D, Antonsson T, Bylund R et al. (1998) Melagatran, a new low molecular weight thrombin inhibitor. Effects on thrombin and fibrinocytic enzymes. Thromb Haemost 79: (in press)
Koch G (1983) Intraclass correlation coefficient. In: Kotz S, Johnson KL (eds) Encyclopedia of statistical science. John Wiley, New York, Vol 4, pp 212–217
Lefkovits J, Topol EJ (1994) Direct thrombin inhibitors in cardiovascular medicine. Circulation 90:1522–1536
Okazaki M, Sakamoto H, Suzuki M et al. (1990) Effects of single and multiple Moxibustions on activity of platelet function, blood coagulation and fibrinolysis in mice. Am J Chin Med 18:77–85
Rowsell HC, Mustard JF (1963) Blood coagulation in some common laboratory animals. Lab Anim Care 13:752–763
Salemink PMJ, Korsten J, de Leeuw P (1994) Prothrombin times and activated partial thromboplastin times in toxicology: a comparison of different blood withdrawal sites for Wistar rats. Comp Haematol Int 4:173–176
Sweeney JD, Novak EK, Reddington M et al. (1990) The RIIIS/J inbred mouse strain as a model for von Willebrand disease. Blood 76:2258–2265
Tabata H, Nakamura S, Matsuzawa T (1995) Some species differences in the false prolongation of prothrombin times and activated partial prothrombin times in toxicology. Comp Haematol Int 5:140–144
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Palm, M., Frankenberg, L., Johansson, M. et al. Evaluation of coagulation tests in mouse plasma. Comparative Haematology International 7, 243–246 (1997). https://doi.org/10.1007/BF02658697
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DOI: https://doi.org/10.1007/BF02658697