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The effect of prolonged treatment with haemin on pluripotent haemopoietic progenitors of normal and hereditarily anaemic Belgrade laboratory (b/b) rats

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Abstract

The Belgrade laboratory (b/b) rat has an intracellular iron and haem deficiency-induced anaemia, due to a mutation affecting intracellular iron transport. Many disturbances in haemopoiesis of the b/b rat have been described, including impaired proliferation and a decreased number of pluripotent and committed progenitor cells in bone marrow as well as expanded granulocytic and erythroid progenitor cell populations in the spleen. In this study, the effects of prolonged treatment with haemin were evaluated on femoral and splenic pluripotent progenitors (spleen colony formingunits day 8, CFU-Sd8) in normal (+/+) and anaemic (b/b) rats. The treatment of b/b rats with haemin did not correct their severe anaemia. However, the abrogation of the proliferative block and an increased proliferative activity of the bone marrow CFU-Sd8 were found, demonstrating that the proliferative block of the b/b rat bone marrow CFU-Sd8 was induced by intracellular iron deficiency, rather than by severe hypoxia. Accordingly, the initially very low number of CFU-Sd8 per femur had increased. The results also revealed an enormously expanded population of splenic CFU-Sd8 in b/b rats, whereas after the haemin treatment a significant decrease in the number of these cells was found. The treatment of +/+ rats with haemin resulted in a significant decrease in the number of femoral CFU-Sd8, but the cycling status of these cells was not affected. In the spleen, the initially low number of CFU-Sd8 was modestly increased after the same treatment. These results imply that the effects of haemin treatment are different in a steady state than in a state of impaired medullar haemopoiesis. It is also evident that for both +/+ and b/b rats there exists an inverse number of CFU-Sd8 in the bone marrow and spleen.

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Authors and Affiliations

  1. Institute for Medical Research, Dr Subotica 4, PO Box 721, 11001, Beograd, Yugoslavia

    Z. Ivanović MD, DSc, G. Jovčić & P. Milenković

  2. Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia

    J. Zarić & Z. Popović

Authors
  1. Z. Ivanović MD, DSc
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  2. J. Zarić
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  3. Z. Popović
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  4. G. Jovčić
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  5. P. Milenković
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Ivanović, Z., Zarić, J., Popović, Z. et al. The effect of prolonged treatment with haemin on pluripotent haemopoietic progenitors of normal and hereditarily anaemic Belgrade laboratory (b/b) rats. Comparative Haematology International 7, 214–219 (1997). https://doi.org/10.1007/BF02658692

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