Abstract
Canola oils at initial stages of processing from different crushing plants were analyzed for phosphatides. The major phospholipid components identified and quantified in refined canola oils were phosphatidic acid and phos-phatidylinositol. Phosphatidic acid was the main phosphorus component identified in solvent extracted canola oil samples. The two-dimensional separation that was used combined classical thin-layer chromatography with quantitation on chromarods in an Iatroscan with a flame ionization detector. Phosphatides quantitated with this procedure ranged from 0.1 to 20 μg with a coefficient of variation of 4.4 to 7.2%. Using the modified procedure, recoveries of better than 90% were obtained for all phospholipids analyzed.
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References
Sonntag N.O.V., inBailey’s Industrial Oil and Fat Products, Vol. I, 4th edn., edited by D. Swern, John Wiley and Sons, New York, 1981, p.49.
Norris F.A., inIbid., Vol. 2, 4th edn., edited by D. Swern, John Wiley and Sons, New York, 1982, p. 255.
Young V.,Chem. Ind. (Lond)18:692 (1978).
Davies M.G., I.M. Zawad and G.S.D. Weir,Scientific and Technological Surveys. No. 112, Leatherhead Food Res. Assoc, Leatherhead, Surrey, U.K., 1979.
Goh S.H., H.T. Khor and P.T. Gee,J. Am. Oil Chem. Soc. 59:298 (1982).
List G.R., CO. Evans, L.T. Black and T.L. Mounts,Ibid. 55:275 (1978).
List G.R., T.L. Mounts, K. Warner and A.J. Heakin,Ibid 55:277 (1978).
List G.R., T.L. Mounts and A.J. Heakin,Ibid. 55:28 (1978).
Wiederman L.H.,Ibid. 58:159 (1981).
Flider F.J., and F.T. Orthoefer,Ibid. 58:270 (1981).
Christie W.W.,J. Chromatogr. 361:396 (1986).
DuPlessis L.M., and H.E. Pretorius,J. Am. Oil Chem. Soc. 60:1261 (1983).
Crane R.T., S.C. Goheen, E.C. Larkin and G. Ananda,Lipids 18:14 (1983).
Min, D.B., Presented at the American Oil Chemists’ Society Short Course on Lipid Oxidation, held on May 13-16, 1987, Biloxi, MO.
Yuen W., and P.C. Kelly,J. Am. Oil Chem. Soc. 57:359 (1980).
Chapman G.W.,Ibid 47:299 (1980).
Kramer J.K.G., E.R. Farnsworth and B.K. Thompson,Lipids 20:536 (1985).
Takemoto Y.,Kawasaki Med. J. 6:1 (1980).
Ackman R.G., inMethods in Enzymology, Vol. 72, Part D, edited by J.M. Lowenstein, Academic Press, New York, 1981, p. 213.
Patterson P.L.,Lipids 20:503 (1985).
Kaimal T.N.E., and N.C. Shanta,J. Chromatogr. 288:111 (1984).
Carter H.E., W.D. Celmer, D.S. Galamos, R.H. Gigg, W.E.M. Lands, J.H. Law, K.L. Mueller, T. Nakayama, H.H. Tomisawa and E. Weber,J. Am. Oil Chem. Soc. 43:461 (1966).
Latta M., and N.A.M. Eskin,J. Agric. Food Chem. 28:1313 (1980).
Lu S.Y., H. Kim, N.A.M. Eskin, S. Johnson and M. Latta,J. Food Sei. 52:173 (1987).
McKillican M.E.,J. Am. Oil Chem. Soc. 43:461 (1966).
Sosulski F., R. Zadernowski and K. Babuchowski,Ibid. 58:561 (1981).
Zadernowski R., and F. Sosulski,Ibid.55:870 (1978).
Hougen, F.W., V.J. Thompson and J.K. Daun, inLecithins, edited by B.F. Szuhaj and G.R. List, Monograph Vol. 12, American Oil Chemists’ Society, p. 79.
Weenink, R.O., and A. P. Tullock,Ibid. 43:327 (1966).
Hvolby A.,J. Am. Oil Chem. Soc. 48:503 (1971).
Larsson J., L. Rydhag and I. Wilton, inProceedings of the 5th International Rapeseed Conference, Vol. 2, Malmo, Sweden, 1978, p. 187.
Kamamoto R., Y. Klada, G. Miyajima and M. Kito,Ibid, 58:1050 (1981).
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Przybylski, R., Eskin, N.A.M. Phospholipid composition of canola oils during the early stages of processing as measured by TLC with flame lonization detector. J Am Oil Chem Soc 68, 241–245 (1991). https://doi.org/10.1007/BF02657617
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DOI: https://doi.org/10.1007/BF02657617