Abstract
The BLV-infected animals were divided into lymphocytotic (9 animals: BLV+PL+) and non lymphocytotic (11 animals: BLV+PL -). BLV-free animals formed a control group (7 animals: BLV-PL-). Flow cytometry immunofluorescence (FACS) and scanning electron microscopy (SEM) were used to evaluate the expression of IgG and IgM molecules on the surface of the lymphocytes of cattle infected with the bovine leukaemia virus (BLV). The percentage of IgM-bearing cells was significantly higher in the BLV+PL+ (75% and 74%) than in BLV+PL (43% and 50%) and BLV-PL (39% and 44%) groups by FACS and SEM respectively. The percentage of IgG-bearing cells was higher in BLV+PL+ (6%) and BLV+PL (8%) compared to BLV-PL (0.7%) determined by FACS and significantly higher in BLV+PL+ (62%) in relation to BLV+PL (17%) and BLV-PL (8%) groups ascertained by SEM. A significantly higher intensity of IgM expression was observed in the BLV+PL+ group by SEM and FACS; a higher intensity of IgG expression in BLV+PL+ group was detected only by SEM. The results suggest that FACS and SEM are complementary. The finding of significant differences in the expression of IgG molecules in BLV+ groups compared with BLV - animals confirms the sensitivity of the SEM method.
Similar content being viewed by others
References
Burny A, Cleuter R, Kettmann R et al. (1988) Bovine leukemia: facts and hypotheses derived from the study of an infectious cancer. Vet Microbiol 17:197–218
De Harven E, Soligo D (1989) Backscattered electron imaging of the colloidal gold marker on cell surfaces. In: Hayat MA (ed) Colloidal gold: principles, methods and applications, Vol. 1, Academic Press, San Diego, pp 230–251
De Harven E, Soligo D, Christensen, H. (1987) Should we be counting immunogold marker particles on cell surfaces with the SEM? J Microsc 146:183–189
Fossum C, Burny A, Portetelle D et al. (1988) Detection of B and T cells, with lectins or antibodies, in healthy and bovine leukemia virus-infected cattle. Vet Immunol Immunopathol 18:269–278
Gatei MH, Lavin MF, Daniel RC (1990) Serum immunoglobulin concentrations in cattle naturally infected with bovine leukemia virus. J Vet Med B 37:575–580
Ishihara K, Ohtani T, Kitagawa H et al. (1980) Clinical studies on bovine leukemia in Japanese black cattle. IV. Serum immunoglobulin concentration in leukemic cattle and those with negative and positive antibodies to bovine leukemia virus. Jap J Vet Sci 42:427–434
Kramme PM, Thomas CB, Schultz D (1994) The contribution of bovine leukaemia virus infected B-cells to the number of circulating B-cells in cattle. Comp Haematol Int 4:96–101
Levkut M, Ponti W, Levkutová, M. et al. (1995a) Monoclonal antibodies recognizing bovine leukocyte antigens used in immunoscanning electron microscopy. Folia Histochem Cytochem 33:179–181
Levkut M, Ponti W, Soligo D et al. (1995b) Expression and quantification of IgG and IgM molecules on the surface of lymphocytes of cattle infected with bovine leukaemia virus. Res Vet Sci 59:45–49
Lydyard P, Grossi C (1993) Development in the immune system. In: Roitt I, Brostoff J, Male D (eds) Immunology, 3rd edn, Mosby, London, pp 11.2–11.5
Mammerickx M, Lorenz RJ, Straub OC et al. (1978) Bovine haematology III. Comparative breed studies on the leukocyte parameters of several European cattle breeds as determined in the common reference laboratory. J Vet Med B 25:257–267
McGroarty RJ, Mills GB, De Harven E (1990) Immunogold labelling of the low-affinity (55kd) IL2 receptor on the surface of IL2 receptor-bearing cultured cells and mitogen-activated peripheral blood lymphocytes. J Leukoc Biol 48:213–229
Pierce KR, Joung MF, Me Arthur NH et al. (1977) Serum immunoglobulin concentration of cattle in a herd with bovine leukosis. Am J Vet Res 38:771–774
Poli G, Turin L, Rocchi M et al. (1996) Reactivity of monclonal antibodies of the B cell panel on PBM from BLV-infected and lymphocytic cows. Vet Immunol Immunopathol 52:295–299
Soligo D, Balercia G, Osculati F et al. (1989) The surface phenotype of lymphatic leukemia cells: An immunoscanning electron microscopy study. Leukemia Lymphoma 1:29–34
Watson JV, Walport MJ (1986) Molecular calibration in flow cytometry with sub-attogram detection limits. J Immunol Methods 93:171–175
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Levkut, M., Ponti, W., Levkutová, M. et al. Immunoanalysis of IgG and IgM molecules on the surface of BLV infected cattle lymphocytes. Comparative Haematology International 7, 152–156 (1997). https://doi.org/10.1007/BF02652593
Issue Date:
DOI: https://doi.org/10.1007/BF02652593