Skip to main content
SpringerLink
Log in

Separation and determination of mono-, Di-, and triglycerides in monoglyceride concentrates

  • Technical
  • Published:
Journal of the American Oil Chemists Society

Summary

Monoglyceride concentrates are quantitatively separated into mono-, di-, and triglyceride components on silica gel columns by an adsorption chromatographic technique. The separated glycerides are determined gravimetrically. The adsorption on silica gel is dependent on the number of hydroxyl groups in the molecule, and the influence of unsaturation and chain length is minimized. Combinations of benzene and ethyl ether are used for elution, thus preventing isomerization, which frequently results when polar solvents such as alcohols are used.

The procedure for the chromatographic separation is simple and straightforward. The equipment used is easily obtainable. The silica gel adsorbent requires only adjustment of its water content before use.

Mixtures of both saturated and unsaturated glycerides and those having different fatty acid chain length have been separated. Known mixtures have also been analyzed. For a known mixture containing 15% tripalmitin, 45% distearin, and 38% monopalmitin the average deviations from the calculated values were 0.4%, 0.2%, and 0.6%, respectively

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Aylward, F., and Wood, D. D. S., Nature,177, 146 (1956).

    Article  CAS  Google Scholar 

  2. Borgström, D., Acta Physiol. Scand.,30, 231–239 (1954).

    Article  Google Scholar 

  3. Deickert, J. W., and Reiser, Raymond, J. Am. Oil Chemists’ Soc.,33, 123–126 (1956).

    Article  Google Scholar 

  4. Dutton, H. J., J. Am. Oil Chemists’ Soc.,32, 652–659 (1955).

    CAS  Google Scholar 

  5. Hamilton, J. G., and Holman, R. T., J. Am. Chem. Soc.,76, 4107–09 (1954).

    Article  CAS  Google Scholar 

  6. Kuhrt, N. H., Welch, Eileen A., Eastman Kodak Co., U. S. Patent 2,727,913 (1955).

  7. Mangold, H. K., Lamp, B. G., and Schlenk, H., J. Am. Chem. Soc.,77, 6070 (1955).

    Article  CAS  Google Scholar 

  8. Pohle, W. D., and Mehlenbacher, V. C., J. Am.Oil Chemists’ Soc.,27, 54–56 (1950).

    Article  CAS  Google Scholar 

  9. Privett, O. S., Ann. Rep. of the Hormel Inst., 1955–1956.

  10. Ravin, L. J., Meyer, R. J., and Higuchi, T., J. Am. Oil Chemists’ Soc.,34, 261–263 (1957).

    CAS  Google Scholar 

  11. Savary, P., and Desnuelle, P., Bull. Soc. Chem. France, 7–8, 936–940 (1954).

Download references

Author information

Authors and Affiliations

  1. Miami Valley Laboratories, The Procter and Gamble Company, Cincinnati, Ohio

    Patricia Quinlin & Herman J. Weiser Jr.

Authors
  1. Patricia Quinlin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Herman J. Weiser Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Quinlin, P., Weiser, H.J. Separation and determination of mono-, Di-, and triglycerides in monoglyceride concentrates. J Am Oil Chem Soc 35, 325–327 (1958). https://doi.org/10.1007/BF02640544

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02640544

Keywords

Search

Navigation