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Analysis of soybean lecithin by thin layer and analytical liquid chromatography

Journal of the American Oil Chemists Society volume 50, pages 513–515 (1973)Cite this article

Abstract

The application of thin layer and analytical liquid chromatography to the analysis of two samples of commercial soybean lecithins is described. A combination of column chromatography and quantitative thin layer chromatography showed that these products consisted of ca. 82% mixture of the major phospholipids of soybeans, phosphatidyl choline, phosphatidyl ethanolamine, and phosphatidyl inositol. The remainder of these products contained essentially the entire spectrum of lipid classes found in soybean oil-some 24 known and unknown glycolipids and phospholipids, in addition to the neutral lipids. Applications of analytical liquid chromatography to these lecithins gave a composition profile of the lipid classes comparable to two-dimensional thin layer chromatography. The potential of this method for the complete analysis of complex lipids, such as soybean lecithins, is indicated.

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Author notes

  1. A. Stolyhwo

    Present address: Department of Food Chemistry and Technology, Technical University Politechnika Gdanska, Gdansk-6, Poland

Authors and Affiliations

  1. The Hormel Institute, University of Minnesota, 55912, Minnesota, Austin

    W. L. Erdahl, A. Stolyhwo & O. S. Privett

Authors
  1. W. L. Erdahl
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  2. A. Stolyhwo
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  3. O. S. Privett
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Erdahl, W.L., Stolyhwo, A. & Privett, O.S. Analysis of soybean lecithin by thin layer and analytical liquid chromatography. J Am Oil Chem Soc 50, 513–515 (1973). https://doi.org/10.1007/BF02640522

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  • DOI: https://doi.org/10.1007/BF02640522

Keywords

  • Lecithin
  • Neutral Lipid
  • Lipid Class
  • Analytical Liquid Chromatography
  • Phosphatidyl Inositol