Analysis of soybean lecithin by thin layer and analytical liquid chromatography
The application of thin layer and analytical liquid chromatography to the analysis of two samples of commercial soybean lecithins is described. A combination of column chromatography and quantitative thin layer chromatography showed that these products consisted of ca. 82% mixture of the major phospholipids of soybeans, phosphatidyl choline, phosphatidyl ethanolamine, and phosphatidyl inositol. The remainder of these products contained essentially the entire spectrum of lipid classes found in soybean oil-some 24 known and unknown glycolipids and phospholipids, in addition to the neutral lipids. Applications of analytical liquid chromatography to these lecithins gave a composition profile of the lipid classes comparable to two-dimensional thin layer chromatography. The potential of this method for the complete analysis of complex lipids, such as soybean lecithins, is indicated.
KeywordsLecithin Neutral Lipid Lipid Class Analytical Liquid Chromatography Phosphatidyl Inositol
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- 1.Food Chemicals Codex National Academy of Science, National Research Council Publication 1143:599 (1962). Official and Tentative Methods of Analysis of the American Oil Chemists' Society, Section J.Google Scholar
- 2.Szuhaj, B.F., Balazs, I.L. and L.D. Williams, Paper 175, World Congress, American Oil Chemists' Society and International Society for Fat Research, Chicago, Ill., September 1970.Google Scholar
- 3.Rouser, R., G. Kritchevsky and A. Yamamoto, in “Lipid Chromatographic Analysis,” Vol. 1, Edited by G.V. Marinetti, Marcel Dekker, Inc., New York, N.Y., 1967, pp. 99–161.Google Scholar
- 5.Privett, O.S., K.A. Dougherty and J.D. Castell, Am. J. Clin. Nutr. 24:1265 (1971).Google Scholar
- 6.Renkonen, O., and P. Varo, in “Lipid Chromatographic Analysis,” Vol. 1, Edited by G.V. Marinetti, Marcel Dekker, Inc., New York, N.Y., 1967, p. 41.Google Scholar
- 7.Dittmer, S.C., and R.L. Lester, J. Lipid Res. 5:126 (1964).Google Scholar
- 8.Skipski, V.P., and M. Barclay, in “Methods of Enzymology,” Edited by J.M. Lowenstein, Academic Press, New York, N.Y., 1969, p. 542.Google Scholar
- 9.Blank, M.L., J.A. Schmit and O.S. Privett, JAOCS 41:371 (1964).Google Scholar
- 10.Stolyhwo, A., and O.S. Privett, J. Chromatogr. Sci. 11:20 (1973).Google Scholar
- 11.Stolyhwo, A., W.L. Erdahl and O.S. Privett, J. Chromatogr. Sci. 11:263 (1973).Google Scholar
- 12.Privett, O.S., K.A. Dougherty, W.L. Erdahl and A. Stolyhwo, JAOCS 50:516 (1973).Google Scholar