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Lymphatic endothelial and smooth-muscle cells in tissue culture

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Summary

Endothelial and smooth-muscle cells from bovine mesenteric lymphatic vessels have been collected and cultured in vitro. The endothelial cells grew as a monolayer exhibiting a “cobblestone” appearance with individual cells tending to be more flattened at confluence than their blood vascular counterparts. Approximately 30% of these cells expressed Factor VIII antigen compared with bovine mesenteric artery or human umbilical-vein endothelium in which the majority of cells were positive. The lymphatic smooth-muscle cells exhibited focal areas of multilayering and were Factor VIII negative. The availability of lymphatic endothelial and smooth-muscle cells in culture will provide a new tool for the investigation of the biological properties of the lymphatic vessels and their role in homeostasis.

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Authors and Affiliations

  1. Department of Pathology, Medical Sciences Building, University of Toronto, M5S 1A8, Toronto, Ontario, Canada

    M. G. Johnston & M. A. Walker

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  1. M. G. Johnston
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  2. M. A. Walker
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Supported by the Medical Research Council of Canada, Grant MA-7925

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Johnston, M.G., Walker, M.A. Lymphatic endothelial and smooth-muscle cells in tissue culture. In Vitro 20, 566–572 (1984). https://doi.org/10.1007/BF02639772

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  • DOI: https://doi.org/10.1007/BF02639772

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