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Effects of VIP on the regulation of mucin secretion in cultured human pancreatic cancer cells (Capan-1)

In Vitro Cellular & Developmental Biology - Animal volume 31, pages 227–233 (1995)Cite this article

Summary

The effects of Vasoactive intestinal peptide (VIP) on mucin secretion in the pancreatic cancer Capan-1 cell line were studied by Enzyme-linked-immunosorbent-assay (ELISA), and by light and electron microscopy using immunocytological methods. During the exponential growth phase, mucins were accumulated in the cytoplasm of cells and slowly exocytosed. In contrast, there was enhanced exocytosis of mucins during the stationary phase when the cells were well-polarized. Moreover, during this phase, VIP induced a dose-dependent rise in mucin content in the extracellular medium. The reaction with anti-MI monoclonal antibodies, which recognize specifically the peptide core of gastric mucins, showed an accumulation of secretion granules near the apex of well-polarized cells together with fusion of the granule and plasma membranes after VIP stimulation. Moreover, mucin exocytosis was stimulated by Pituitary adenylate cyclase activating polypeptide (PACAP) and secretin. It was also increased after forskolin treatment suggesting that this mechanism was cAMP-dependent. Our results suggested that exocytosis of mucins could be under the control of VIP in pancreatic duct cells of the Capan-1 cell line.

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  1. Laboratoire de Biologie Cellulaire, Université Paul Sabatier, 38 rue des Trente-Six Ponts, 31400, Toulouse, France

    Etienne Hollande, Marjorie Fanjul & Serge Claret

  2. Unité de Recherches de Diabétologie et d’Etudes Radioimmunologiques des Hormones Protéiques, INSERM U55, Hôpital Saint-Antoine, 184 rue du Faubourg Saint-Antoine, 75571, Paris, France

    Marie-Elizabeth Forgue-Lafitte & Jacques Bara

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  1. Etienne Hollande
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  2. Marjorie Fanjul
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  3. Serge Claret
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  4. Marie-Elizabeth Forgue-Lafitte
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  5. Jacques Bara
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Hollande, E., Fanjul, M., Claret, S. et al. Effects of VIP on the regulation of mucin secretion in cultured human pancreatic cancer cells (Capan-1). In Vitro Cell Dev Biol - Animal 31, 227–233 (1995). https://doi.org/10.1007/BF02639438

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  • DOI: https://doi.org/10.1007/BF02639438

Key words

  • human pancreas
  • cancer
  • mucins
  • VIP
  • exocytosis