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Incorporation of exogenous docosahexaenoic acid into various bacterial phospholipids

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Journal of the American Oil Chemists’ Society

Abstract

Incorporation of exogenous docosahexaenoic acid (DHA) into bacterial phospholipids was examined as a method for DHA-linked phospholipid production. The cultivation of 23 bacterial strains in medium with DHA showed that an eicosapentaenoic acid-producing bacteriumShewanella sp. strain SCRC-2738 (strain SCRC-2738),Bacillus subtilis W23,B. cereus, an Antarctic marine bacterium strain S-7 (strain S-7), photosynthesis bacterium (PSB)Rhodopseudomonas capsulatus utilized for the production of larval marine fish,Pseudomonas aeruginosa, Staphylococcus aureus, Serratia marcescens andEscherichia coli K12 all incorporated DHA into their polar lipids. The polar lipids of the strain SCRC-2738, strain S-7, PSB andE. coli K12 were identified to be phospholipids. DHA was localized at thesn-2 position in the phospholipids of the four strains. Incorporation of exogenous DHA into their phospholipids produced an increase in saturated fatty acids and a decrease in monounsaturated fatty acids exceptE. coli K12. The strain SCRC-2738 incorporated the largest amount of DHA into their phospholipids among the tested bacterial strains in this study: DHA was 16% of the total fatty acids in the phosphatidylethanolamine (PE) and 29% in the phosphatidylglycerol (PG). In the PSB, incorporated DHA was 12% of the total fatty acids in the PE, 10% in the PG and phosophatidylcholine so that the PSB was nutritionally fortified.

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References

  1. Lands, W.E.M., inFish and Human Health, Academic Press, Inc., London, 1986, pp. 7–87.

    Google Scholar 

  2. Anderson, G.J., W.E. Connor and J.D. Corliss,Pediatr. Res. 27:89 (1990).

    Article  PubMed  CAS  Google Scholar 

  3. Yazawa, K., and H. Kageyama,Yukagaku (in Japanese)40:974 (1991).

    CAS  Google Scholar 

  4. Yazawa, K., and Y. Masuzawa,Ibid. (in Japanese)40:845 (1991).

    CAS  Google Scholar 

  5. Nakajima, M., Y. Sohda, K. Kashima, H. Kudo and A. Miyamoto, Japanese Patent 76/91213 (1976).

  6. Noguchi, Y., Japanese Patent 86/129191 (1986).

  7. Hosokawa, M., H. Ohshima, H. Kohno, K. Takahashi, M. Hatano and S. Odashima,Nippon Suisan Gakkaishi (in Japanese)59:309 (1993).

    CAS  Google Scholar 

  8. Totani, Y., and S. Hara,J. Am. Oil Chem. Soc. 68:848 (1991).

    CAS  Google Scholar 

  9. Kageyama, H., K. Sakai, Y. Isoda and Y. Nishizawa, Japanese Patent 88/164852 (1988).

  10. Nishizawa, Y., Y. Isoda, H. Kageyama and K. Sakai, Japanese Patent 89/50890 (1989).

  11. Fukuda, H., H. Hibino and O. Nakachi, Japanese Patent 89/131189 (1989).

  12. Hibino, H., N. Fukuda, O. Nakachi, S. Sakurai, K. Asahi and N. Fukunaga, Japanese Patent 89/160989 (1989).

  13. Silbert, D.F., T.M. Ulbright and J.L. Honegger,Biochemistry 12:164 (1973).

    Article  PubMed  CAS  Google Scholar 

  14. Smittle, R.B., S.E. Gilliland, M.L. Speck and W.M. Walter, Jr.,Appl. Microbiol. 27:738 (1974).

    PubMed  CAS  Google Scholar 

  15. Clejan, S., A.A. Guffanti, L.H. Falk and T.A. Krulwich,Biochim. Biophys. Acta 932:43 (1988).

    Article  PubMed  CAS  Google Scholar 

  16. Williams, J.P., E. Maissan, K. Mitchell and M.U. Khan,Plant Cell Physiol. 31:495 (1990).

    CAS  Google Scholar 

  17. Hallaq, H., A. Sellamayer, T.W. Smith and A. Leaf,Proc. Natl. Acad. Sci. USA 87:7834 (1990).

    Article  PubMed  CAS  ADS  Google Scholar 

  18. Yazawa, K., K. Araki, K. Watanabe, C. Ishikawa, A. Inoue, N. Numao, K. Kondo, S. Watabe and K. Hashimoto,Nippon Suisan Gakkaishi 54:1825 (1988).

    Google Scholar 

  19. Kobayashi, M., K. Mochida and A. Okuda,Bull. Jap. Soc. Sci. Fish. 33:657 (1967).

    CAS  Google Scholar 

  20. Kaufmann, N., H.H. Reidl, J.R. Golecki, A.F. Garcia and G. Drews,Arch. Microbiol. 131:313 (1982).

    Article  CAS  Google Scholar 

  21. Flammamm, H.T., and J. Weckesser,J. Bacteriol. 159:191 (1984).

    Google Scholar 

  22. Hirayama, O.,Agr. Biol. Chem. 32:34 (1968).

    CAS  Google Scholar 

  23. Yone, Y., inYougyo to Shiryoshishitsu (in Japanese), Koseisha-Koseikaku, Tokyo, 1978, pp. 43.

    Google Scholar 

  24. Folch, J., W. Lee and G.H.S. Stanley,J. Biol. Chem. 226:497 (1957).

    PubMed  CAS  Google Scholar 

  25. Haverkate, F., and L.L.M. van Deenen,Biochim. Biophys. Acta 106:78 (1965).

    PubMed  CAS  Google Scholar 

  26. Black, P.N., S.F. Kianian, C.C. DiRusso and W.D. Nunn,J. Biol. Chem. 260:1780 (1985).

    PubMed  CAS  Google Scholar 

  27. Cronan, Jr., J.E.,J. Bateriol. 159:773 (1984).

    CAS  Google Scholar 

  28. Rock, C.O., and S. Jackowski,J. Biol. Chem. 260:12720 (1985).

    PubMed  CAS  Google Scholar 

  29. Okuyama, H.,Seikagaku (in Japanese)47:999 (1975).

    PubMed  CAS  Google Scholar 

  30. Applegate, K.R., and J.A. Glomset,J. Lipid Res. 27:658 (1986).

    PubMed  CAS  Google Scholar 

  31. Litman, B.J., E.N. Lewis and I.W. Levin,Biochemistry 30:313 (1991).

    Article  PubMed  CAS  Google Scholar 

  32. Deese, A.J., E.A. Dratz, F.W. Dahlquist and M.R. Radcly,Ibid. 20:6420 (1981).

    Article  PubMed  CAS  Google Scholar 

  33. Cevc, G.,Ibid. 30:7186 (1991).

    Article  PubMed  CAS  Google Scholar 

  34. Bremer, J., and H. Osmundsen, inFatty Acids Metabolism and Its Regulation, edited by S. Numa, Elsevier, Amsterdam, 1984, pp. 113–154.

    Google Scholar 

  35. Dommes, V., C. Baumgart and W.H. Kunau,J. Biol. Chem. 256:8259 (1981).

    PubMed  CAS  Google Scholar 

  36. Mizugaki, M., T. Nishimaki, H. Yamamoto, S. Nishimura, M. Sagi and H. Yamanaka,J. Biochem. 91:1453 (1982).

    PubMed  CAS  Google Scholar 

  37. Jaworski, J.G., inThe Biochemistry of Plants, Vol. 9, Academic Press, Inc., 1987, pp. 159–174.

  38. Giroud, C., and W. Eichenberger,Plant Cell Physiol. 30:121 (1989).

    CAS  Google Scholar 

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Authors and Affiliations

  1. Sagami Chemical Research Center, 229, Kanagawa, Nishi-Ohnuma 4-4-1, Sagamihara, Japan

    Kazuo Watanabe, Chikako Ishikawa, Hitomi Inoue, Deng Cenhua, Kazunaga Yazawa & Kiyosi Kondo

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  1. Kazuo Watanabe
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  2. Chikako Ishikawa
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  3. Hitomi Inoue
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  4. Deng Cenhua
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  5. Kazunaga Yazawa
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  6. Kiyosi Kondo
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Watanabe, K., Ishikawa, C., Inoue, H. et al. Incorporation of exogenous docosahexaenoic acid into various bacterial phospholipids. J Am Oil Chem Soc 71, 325–330 (1994). https://doi.org/10.1007/BF02638061

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  • DOI: https://doi.org/10.1007/BF02638061

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