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In vivo labeling of brain phospholipids by long-chain fatty acids: Relation to turnover and function

  • In Vivo Assessment of Fatty Acid Status
  • Published:
Lipids

Abstract

An experimental method and model are described to quantitate kinetics ofin vivo incorporation of fatty acids (FA) into stable brain phospholipids. When a radiolabeled long-chain FA is injected intravenously in a rat, it rapidly equilibrates with brain FA-CoA, the precursor pool for phospholipids. As different labeled FA enter differentsn positions of specific phospholipids, a combination of labels can be used to investigate roles of different phospholipids in brain function and structure. By taking into account dilution λ of specific activity of brain FA-CoA, compared with specific activity of FA in plasma, half-lives of FA in individual brain phospholipids can be calculated. Values for λ less than 0.02 suggest marked recycling, and give half-lives two orders of magnitude smaller than literature values. A half-life of arachidonate in phosphatidyli-nositol of 0.66 h (turnover=105%h) is consistent with active participation of this FA in phospholipase A2mediated signal transduction.

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Abbreviations

FA:

fatty acid

i.v.:

intravenous

NBM:

nucleus basalis of Meynert

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PI:

phosphatidylinositol

rCBF:

regional cerebral blood flow

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Authors and Affiliations

  1. Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, 20892, Bethesda, Maryland

    Stanley I. Rapoport

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  1. Stanley I. Rapoport
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Rapoport, S.I. In vivo labeling of brain phospholipids by long-chain fatty acids: Relation to turnover and function. Lipids 31, S97–S101 (1996). https://doi.org/10.1007/BF02637059

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