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The unsolved problems of triglyceride analysis

  • Technical
  • T. P. Hilditch Symposium On Analysis Of Natural Fat Triglycerides. IV
  • Published:
Journal of the American Oil Chemists Society

Abstract

The modern techniques of countercurrent distribution between solvents, GLC and TLC (especially on silver nitrate impregnated silica gel), and enzymic hydrolysis have greatly simplified and speeded up the analysis of triglyceride mixtures, such as are found in natural fats. The application of these methods has provided data for the formulation of a number of new theoretical descriptions of the fatty acid distributions found in fats.

But it is clear that neither the experimental techniques, nor the theories of glyceride structure arrived at with their aid, are entirely satisfactory. On the one hand analytical methods are inadequate for such fats as the nut oils; and in no case can the accuracy of the analysis be regarded as complete. On the other hand present theories have yet to accommodate minor components, enantiomorphic forms, and recent evidence concerning the biosynthesis of natural triglycerides.

Attention is drawn to some of the outstanding problems still facing those concerned with the analysis of triglyceride mixtures; and an attempt it made to indicate the direction in which solutions to these problems may be sought.

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References

  1. Korn, E. D., J. Lipid Res.5, 352 (1964).

    PubMed  CAS  Google Scholar 

  2. Coleman, M. H., in “Advances in Lipid Research,” ed. R. Paoletti and D. Kritchevsky, 1963, Vol. 1. p. 1.

  3. James, A. T., in “Methods of Biochemical Analysis,” ed. D. Glick, 1960, Vol. 8, p. 1.

  4. Schlenk, H., and J. L. Gellerman, Anal. Chem.32, 1412 (1960).

    Article  CAS  Google Scholar 

  5. Johnston, P. V., and B. I. Roots, J. Lipid Res.5, 477 (1964).

    PubMed  CAS  Google Scholar 

  6. Stoffel, W., F. Chu and E. H. Ahrens, Anal. Chem.31, 307 (1959).

    Article  CAS  Google Scholar 

  7. Fontell, K., R. T. Holman and G. Lambertsen, J. Lipid Res.,1, 391 (1960).

    PubMed  CAS  Google Scholar 

  8. Morris, L. J., R. T. Holman and K. Fontell, JAOCS37, 323 (1960).

    CAS  Google Scholar 

  9. Morris, L. J., R. T. Holman and K. Fontell, J. Lipid Res.1, 412 (1960).

    PubMed  CAS  Google Scholar 

  10. Kartha, A.R.S., JAOCS30, 326 (1953).

    CAS  Google Scholar 

  11. VanderWal, R. J.,Ibid. 37, 18 (1960).

    Article  CAS  Google Scholar 

  12. Gunstone, F. D., Chem. Ind. (London) —— 1214 (1962).

    Google Scholar 

  13. Hilditch, T. P., and C. H. Lea, J. Chem. Soc. —— 3106 (1927).

    Article  Google Scholar 

  14. Hilditch, T. P., and P. N. Williams, “The Chemical Constitution of Natural Fats,” 4th Ed. Chapman & Hall London. 1964.

  15. Dutton, H. J., and J. A. Cannon, JAOCS33, 46 (1956).

    CAS  Google Scholar 

  16. De Vries, B., Chem. Ind. (London) —— 1049 (1962).

    Google Scholar 

  17. Barrett, C. B., M. S. J. Dallas and F. B. Pedley,Ibid. —— 1050 (1962).

    Google Scholar 

  18. Kuksis, A., and H. J. McCarthy, Can. J. Biochem. Physiol.40, 679 (1962).

    PubMed  CAS  Google Scholar 

  19. Kaufmann, H. P., and H. Wessels, Fette Scifen Anstrichmittel66, 81 (1964).

    Article  CAS  Google Scholar 

  20. Vereshchagin, A. G., Biokhimiya27, 866 (1962).

    CAS  Google Scholar 

  21. Mattson, F. H., and L. W. Beck, J. Biol. Chem.214, 115 (1955).

    PubMed  CAS  Google Scholar 

  22. Savary, P., and P. Desnuelle, Compt. Rend.240, 2571 (1955).

    CAS  Google Scholar 

  23. Hofmann, A., J. Lipid Res.3, 391 (1962).

    CAS  Google Scholar 

  24. Schonheyder, F., and K. Volqvartz, Acta Physiol. Scand.10, 62 (1945).

    Article  CAS  Google Scholar 

  25. Desnuelle, P., and M. J. Constantin. Res. Commun. 2e Congr. Int. Biochem., Paris, 1952, p. 153.

  26. Sager, C. A., Biochem. Z.321, 44 (1950).

    PubMed  CAS  Google Scholar 

  27. Fritz, P. J., and P. Melius, Can. J. Biochem. Physiol.41, 719 (1963).

    PubMed  CAS  Google Scholar 

  28. Entressangles, B., L. Pasero, P. Savary, P. Desnuelle and L. Sarda, Bull. Soc. Chim. Biol.43, 583 (1961).

    Google Scholar 

  29. Clement, G., J. Clement and J. Bezard, Biochem. Biophys. Res. Comm.8, 238 (1962).

    Article  PubMed  CAS  Google Scholar 

  30. Coleman, M. II., JAOCS40, 568 (1963).

    CAS  Google Scholar 

  31. Bottino, N. R., G. Vanderburg, and R. Reiser, in preparation.

  32. Subbaram, M. R., M. M. Chakrabarty, C. G. Youngs and B. M. Craig,Ibid. 41, 691 (1964).

  33. Chapman, D., A. Crossley and A. C. Davis, J. Chem. Soc. —— 1502 (1957).

    Article  Google Scholar 

  34. Brockerhoff, H., J. Lipid Res.6, 10 (1965).

    PubMed  CAS  Google Scholar 

  35. Brockerhoff, H., Arch. Biochem. Biophys., in press.

  36. Coleman, M. H., unpublished.

  37. Mattson, F. H., and R. A. Volpenhein, J. Lipid Res.5, 374 (1964).

    PubMed  CAS  Google Scholar 

  38. Von Rudloff, E., Can. J. Chem.34, 1413 (1956).

    Article  Google Scholar 

  39. Morris, L. J., in preparation.

  40. Sweeley, C. C., R. Bentley, M. Makita and W. W. Wells, J. Am. Chem. Soc.85 2497 (1963).

    Article  CAS  Google Scholar 

  41. Schlenk. W., “Festschrift Carl Wurster zum 60 Geburstag,” (1960), p. 105.

  42. Brockerhoff, H., personal communication to the author.

  43. Brockerhoff, H., R. J. Hoyle and N. Wolmark, Federation Proc.24, 662, 1965.

    Google Scholar 

  44. Kaufmann, H. P., and H. Wessels, Fette Seifen Anstrichmittel66, 13 (1964).

    Article  CAS  Google Scholar 

  45. Barrett, C. B., M. S. J. Dallas and F. B. Padley, JAOCS40, 580 (1963).

    CAS  Google Scholar 

  46. Youngs, C. G., and M. R. Subbaram,Ibid. 41, 218 (1964).

    CAS  Google Scholar 

  47. Jurriens, G., and A. C. J. Kroesen,Ibid. 42, 9 (1965).

    Article  PubMed  CAS  Google Scholar 

  48. Youngs, C. G.,Ibid. 38, 62 (1961).

    CAS  Google Scholar 

  49. Subbaram, M. R., and C. G. Youngs,Ibid. 41, 445 (1964).

    CAS  Google Scholar 

  50. Subbaram, M. R., and C. G. Youngs,Ibid. 41, 595 (1964).

    CAS  Google Scholar 

  51. Kuksis, A., and H. J. McCarthy, Can. J. Biochem. Physiol.40, 679 (1962).

    PubMed  CAS  Google Scholar 

  52. Coleman, M. H., JAOCS38, 685 (1961).

    Article  CAS  Google Scholar 

  53. Gander, G. W., R. G. Jensen and J. Sampugna, J. Dairy Sci.44, 1980 (1961).

    Article  CAS  Google Scholar 

  54. Litchfield, C., Margaret Farquhar and R. Reiser, JAOCS41, 588 (1964).

    CAS  Google Scholar 

  55. Savary, P., and P. Desnuelle, Arch. Sci. Biol.39, 689 (1955).

    CAS  Google Scholar 

  56. Achaya, K. T., B. M. Craig and C. G. Youngs, JAOCS41, 783 (1964).

    CAS  Google Scholar 

  57. Coleman, M. H.,Ibid. 41, 247 (1964).

    CAS  Google Scholar 

  58. Kartha, A. R. S., J. Sci. Food Agric.14, 515 (1963).

    Article  CAS  Google Scholar 

  59. Galloppini, C., and G. Lotti, Chim. Ind. (Milan)45, 812 (1963).

    CAS  Google Scholar 

  60. Harlow, R. D., C. Litchfield, H. C. Fu and R. Reiser, JAOCS42, 747–750 (1965).

    Article  CAS  Google Scholar 

  61. Litchfield, C., and R. Reiser,Ibid. 42, 757–762 (1965).

    Article  CAS  Google Scholar 

  62. James, A. T., op. cit. (3), p. 41.

  63. Coleman, M. H., JAOCS, in press.

  64. Claxton, G., J. Chromatog.2, 136 (1959).

    Article  CAS  Google Scholar 

  65. Haahti, E., T. Nikkari and K. Juva, Acta Chem. Scand.17, 538 (1963).

    Article  CAS  Google Scholar 

  66. James, A. T., J. R. Ravenhill and R. P. W. Scott. Chem. Ind. (London), (1964), p. 746.

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Authors and Affiliations

  1. Unilever Research Laboratory, Colworth House, Sharnbrook, Bedford, England

    M. H. Coleman

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Coleman, M.H. The unsolved problems of triglyceride analysis. J Am Oil Chem Soc 42, 1040–1046 (1965). https://doi.org/10.1007/BF02636901

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