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Rapid colorimetric determination of free fatty acids

Journal of the American Oil Chemists Society volume 53, pages 470–472 (1976)Cite this article

Abstract

In 1964, a method was described for the determination of free fatty acids (FFAs) in vegetable oil. This paper describes an expansion of that work, improving the sensitivity and reproducibility of the method, as well as examination of solubilities of the copper soaps as a function of chain length and unsaturation. Involvement of the micellar structure was reviewed. Finally, a procedure is described that permits very rapid determination of FFA at the 2.0–14.0 µmol (0.5–4.0 mg oleic acid) level, and the results with several oils are given. Particular attention was given to evaluation of solvent systems which would extract the copper complexes.

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References

  1. Ayers, C.W., Anal. Chim. Acta 15:77 (1956).

    Article  CAS  Google Scholar 

  2. Baker, D., Cereal Chem. 38:47 (1961).

    CAS  Google Scholar 

  3. Baker, D., JAOCS 41:21 (1964).

    Google Scholar 

  4. Bains, G.S., S.V. Rao, and D.S. Bhatia, Ibid. 41:832 (1964).

    Google Scholar 

  5. Markley, K.S., Editor, “Fatty Acids,” 2nd edition, Interscience Publishers, Inc., New York, NY, 1960, Part 1, p. 634.

    Google Scholar 

  6. Koenig, A.E., J. Am. Chem. Soc. 36:951 (1914).

    Article  CAS  Google Scholar 

  7. Iwayama, Y., J. Pharm. Soc. Japan 79:552 (1959).

    CAS  Google Scholar 

  8. Duncombe, W.G., Biochem. J. 88:7 (1963).

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Agricultural Chemistry, Oregon State University, 97331, Corvallis, Oregon

    Robert R. Lowry & Ian J. Tinsley

Authors
  1. Robert R. Lowry
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  2. Ian J. Tinsley
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Additional information

Technical Paper No. 4036, Oregon Agricultural Experiment Station.

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Lowry, R.R., Tinsley, I.J. Rapid colorimetric determination of free fatty acids. J Am Oil Chem Soc 53, 470–472 (1976). https://doi.org/10.1007/BF02636814

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  • DOI: https://doi.org/10.1007/BF02636814

Keywords

  • Free Fatty Acid
  • Oleic Acid
  • Isobutyl Alcohol
  • Cupric Acetate
  • Saturated Free Fatty Acid