Lipase fromCandida rugosa was modified with several hydrophobic modifiers before being adsorbed onto organic polymer beads. The effects of different enzyme modifiers, supports, solvents, reaction temperatures, fatty acids, and alcohols on the activity of the immobilized enzyme were investigated. The immobilized lipases were good biocatalysts for esterification reactions in organic solvents. They exhibited high activities in all solvents tested, including polar solvents. The activity seemed to depend on the type of support rather than on the modifier of the enzyme. The medium polar support, XAD7, appeared to be the best for the modified lipases. The immobilized lipase favored the medium-chain fatty acids rather than the long-chain fatty acids as acyl donors. The alcohol selectivity of the enzyme was unchanged upon immobilization. The native and immobilized lipases favored the short-chain and terpene alcohols as nucleophiles.
This is a preview of subscription content, access via your institution.
Choo, S., and J.S. Rhee,Biotechnol. Bioengin. 41:204 (1993).
Ruckenstein, E., and X. Wang, Ibid.:821 (1993).
Basri, M., K. Ampon, W.M.Z. Wan Yunus, C.N.A. Razak and A.B. Salleh,J. Chem. Technol. Biotechnol. 59:37 (1994).
Basri, M., A.B. Salleh, K. Ampon, W.M.Z. Wan Yunus and C.N.A. Razak,Biocatalysis 4:313 (1991).
Ampon, K., A.B. Salleh, F. Salam, W.M.Z. Wan Yunus, C.N.A. Razak and M. Basri,Enzyme Microb. Technol. 13:597 (1991).
Basri, M., K. Ampon, W.M.Z. Wan Yunus, C.N.A. Razak and A.B. Salleh,J. Am. Oil Chem. Soc. 69:579 (1992).
Wofsy, M., and S.J. Singer,Biochem. 21:104 (1963).
Hazra, A.K., S.P. Chock and R.W. Albers,Analyt. Biochem. 137:437 (1985).
Fields, R.,Biochem. J. 124:581 (1972).
Malcata, F.X., H.R. Reyes, H.S. Garcia, C.J.G. Hill and C.H. Amundson,J. Am. Oil Chem. Soc. 67:890 (1990).
Laane, C., S. Boeren, K. Vos and C. Veeger,Biotechnol. Bioengin. 30:81 (1986).
Basri, M., K. Ampon, W.M.Z. Wan Yunus, C.N.A. Razak and A.B. Salleh,Appl. Biochem. Biotechnol. 48:158 (1994).
Gorman, L.A., and J.S. Dordick,Biotechnol. Bioengin. 39:392 (1992).
Bar-Eli, A., and E. Katchalski,J. Biol. Chem. 238:1690 (1963).
Glassmeyer, C.K., and J.D. Ogle,Biochem. 10:786 (1971).
Koshiro, S., K. Sonomoto, A. Tanaka and S. Fukui,J. Biotechnol. 2:47 (1985).
Kawamoto, T., K. Sonomoto and A. Tanaka,Biocatalysis I:137 (1987).
Cabezas, M.J., C. Del Campo, E. Llama and J.V. Sinisterra,J. Molec. Catalysis 71:261 (1992).
Hills, M., I. Kiewitt and K. Mukherjee,J. Am. Oil Chem. Soc. 67:561 (1990).
Pan, S., T. Kawamoto, T. Fukui, K. Sonomoto and A. Tanaka,Appl. Microbiol. Biotechnol. 34:47 (1990)
About this article
Cite this article
Basri, M., Ampon, K., Wan Yunus, W.M.Z. et al. Enzymic synthesis of fatty esters by hydrophobic lipase derivatives immobilized on organic polymer beads. J Am Oil Chem Soc 72, 407–411 (1995). https://doi.org/10.1007/BF02636079
- immobilized enzyme
- lipase modification