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Effect of juvenile hormone on the embryogenesis of a polyembryonic wasp, copidosoma floridanum, in vitro

  • Growth, Differentiation And Senescence
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Summary

Single two-cell-stage embryos of a polyembryonic waspCopidosoma floridanum cultured in 20 µl droplets of culture medium developed to morulae at the same developmental rate as those in host eggs, but the subsequent development into polymorulae was inferior. This inferior development was markedly improved by addition of juvenile hormone (I, II, or III) or its analogues to the culture medium in a concentration-dependent manner.

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References

  1. Baehrecke, E. H.; Aiken, J. M.; Dover, B. A., et al. Ecdysteroid induction of embryonic morphogenesis in a parasitic wasp. Dev. Biol. 158:275–287; 1993.

    Article  PubMed  CAS  Google Scholar 

  2. Bergot, B. J.; Baker, F. C.; Cerf, D. C., et al. Qualitative and quantitative aspects of juvenile hormone titers in developing embryos of several insect species: Discovery of a new JH-like substance extracted from eggs ofManduca sexta. In: Pratt, G. E.; Brooks, G. T., eds. Developments in endocrinology 15, juvenile hormone biochemistry. Amsterdam, Netherlands: Elsevier/North Holland; 1981:33–45.

    Google Scholar 

  3. Grossniklaus-Burgin, C.; Lanzrein, B. Qualitative and quantitative analyses of juvenile hormone and ecdysteroids from the egg to the pupal molt inTrichoplusia ni. Arch. Insect Biochem. Physiol. 14:13–30; 1990.

    Article  PubMed  CAS  Google Scholar 

  4. Grossniklaus-Burgin, C.; Lanzrein, B. Endocrine interrelationship between the parasitoidChelonus sp. and its hostTrichoplusia ni. Arch. Insect Biochem. Physiol. 14:201–216; 1990.

    Article  PubMed  CAS  Google Scholar 

  5. Hartman, R.; Jendrsczok, C.; Peter, M. G. The occurrence of a juvenile hormone binding protein and in vitro synthesis of juvenile hormone by the serosa ofLocusta migratoria embryos. Roux’s Arch. Dev. Biol. 196:347–355; 1987.

    Article  Google Scholar 

  6. Iwabuchi, K. Early embryonic development of a polyembryonic wasp,Litomastix maculata Ishii, in vivo and in vitro. Appl. Entomol. Zool. 26:563–570; 1991.

    Google Scholar 

  7. Kawasaki, K.; Ikeuchi, M.; Hidaka, T. Laboratory rearing method forAcanthoplusia agnata (Lepidoptera: Noctuidae) without changing of artificial diet. Jpn. J. Appl. Entomol. Zool. 31:78–80; 1987.

    Google Scholar 

  8. Mitsuhashi, J.; Inoue, H. Obtainment of a continuous cell line from the larval fat bodies of the mulberry tiger moth,Spilosoma imparilis (Lepidoptera: Arctiidae). Appl. Entomol. Zool. 23:488–490; 1988.

    Google Scholar 

  9. Nenon, J.-P. Culture in vitro des embryons d’un Hymenoptere endoparasite polyembryonnaire:Ageniaspis fuscicollis (=Encyrtus fuscicollis). Role des hormones de synthese. C. R. Acad. Sci. Paris. D. 274:3299–3302; 1972.

    CAS  Google Scholar 

  10. Nenon, J.-P. Culture in vitro des larves d’un Hymenoptere endoparasite polyembryonnaire:Ageniaspis fuscicollis. Role des hormones de synthese. C. R. Acad. Sci. Paris. 274:3409–3412; 1972.

    CAS  Google Scholar 

  11. Slama, K. Pharmacology of insect juvenile hormones. In: Kerkut, G. A.; Gilbert, L. I., eds. Comprehensive insect physiology, biochemistry and pharmacology. Vol. 11. New York: Pergamon Press; 1985:357–394.

    Google Scholar 

  12. Strand, M. R.; Baehrecke, E. H.; Wong, E. A. The role of host endocrine factors in the development of polyembryonic parasitoids. Biol. Contr. 1:144–152; 1991.

    Article  Google Scholar 

  13. Strand, M. R.; Goodman, W. G.; Baehrecke, E. H. The juvenile hormone titer ofTrichoplusia ni and its potential role on embryogenesis of the polyembryonic waspCopidosoma floridanum. Insect Biochem. 21:205–214; 1991.

    Article  CAS  Google Scholar 

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  1. Tokyo University of Agriculture and Technology, Saiwai-cho, Fuchu, 183, Tokyo, Japan

    K. Iwabuchi

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  1. K. Iwabuchi
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Iwabuchi, K. Effect of juvenile hormone on the embryogenesis of a polyembryonic wasp, copidosoma floridanum, in vitro. In Vitro Cell Dev Biol - Animal 31, 803–805 (1995). https://doi.org/10.1007/BF02634123

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  • DOI: https://doi.org/10.1007/BF02634123

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