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Vitronectin secretion by hepatic and non-hepatic human cancer cells

  • Growth, Differentiation And Senestence
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Summary

Thirty-eight human cancer cell lines and subclones derived from 12 different organs were screened for vitronectin secretion in their culture media. By immunoblotting analysis we detected high secretion by three out of five hepatoma cell lines tested but no secretion by the others. In addition, significant secretion was observed in seven non-hepatic cancer cell lines and subclones derived from the cervix, lung, and pancreas. These vitronectin-secreting cells included PLC/PRF/5, HuH-6 #5, HuH-7, HeLa S3, HeLa · P3 #2, #3, #6, #8, A549, and MIAPaCa-2. The results were further confirmed by quantitative analysis using sandwich enzyme-linked immunoassay, and activity analysis of cell attachment promotion on Western blotted filters.

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Authors and Affiliations

  1. Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, 2-120-3, Nakamura-cho, Minami-ku, 232, Yokohama, Japan

    Hidetaro Yasumitsu, Noriko Seo, Emi Misugi, Hatsuė Morita, Kaoru Miyazaki & Makoto Umeda

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  1. Hidetaro Yasumitsu
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  2. Noriko Seo
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  3. Emi Misugi
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  4. Hatsuė Morita
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  5. Kaoru Miyazaki
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  6. Makoto Umeda
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Yasumitsu, H., Seo, N., Misugi, E. et al. Vitronectin secretion by hepatic and non-hepatic human cancer cells. In Vitro Cell Dev Biol - Animal 29, 403–407 (1993). https://doi.org/10.1007/BF02633989

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  • DOI: https://doi.org/10.1007/BF02633989

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