Summary
Human non-autocrine neuroblastoma cells SK-N-SH and LF require serum for proliferation in vitro. We wished to determine the role of serum-borne insulin-like growth factor I (IGF-I) as mitogen for these cells. Introduction of the monoclonal antibodyα-IR3 against human IGF-I receptor reduced proliferation in the presence of fetal bovine serum (FBS). IGF-I (5 nM) was as effective as FBS (10%) in stimulating proliferation. Porcine insulin mimicked the effects of IGF-I, but at a 1000-fold higher concentration. The antibodyα-IR3 reduced growth stimulated by IGF-I more effectively than growth stimulated by insulin. Thus, proliferation of human non-autocrine neuroblastoma cells can be effectively manipulated by exogenous IGF-I.
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Burke, T.W., Vuk-Pavlović, S. Insulin-like growth factor-I is a serum component stimulating growth of human neuroblastoma. In Vitro Cell Dev Biol - Animal 29, 391–394 (1993). https://doi.org/10.1007/BF02633987
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DOI: https://doi.org/10.1007/BF02633987