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Histochemical identification of cultured cells from human endometrium

In Vitro volume 20, pages 25–32 (1984)Cite this article

Summary

Histochemical techniques have been applied to the identification of cell types cultured from human endometrium. Previous work from this laboratory characterized two principtal cell types found in cultures of endometrium: a mature epithelial cell and another cell which was classified as the endometrial stromal cell based on light and electron microscopy. In this report we compare the histochemical staining of endometrial tissue in frozen sections to that of cultured cells. These results confirm the epithelial and stromal nature of the respective cell types. Several markers were found that could distinguish between cells of epithelial and stromal origin. The enzymes alkaline phosphatase, γ-glutamyltranspeptidase, peroxidase, and β-glucuronidase were localized in glandular and surface epithelia in frozen sections and in colonies of epithelial cells in culture. Stroma in frozen sections and cultured stromal cells contained leucine aminopeptidase and fibronectin. Epithelial sections and in culture could also be distinguished from cells of stromal origin by preferential binding of lotus and peanut lectin. Several other markers were found in both endometrial epithelium and stroma.

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Affiliations

  1. Department of Pathology, University of North Carolina at Chapel Hill, 27514, Chapel Hill, North Carolina

    Karen G. Nelson, Jane L. Martin & David G. Kaufman

  2. Cancer Research Center, University of North Carolina at Chapel Haill, 27514, Chapel Hill, North Carolina

    Jill M. Siegfried & David G. Kaufman

Authors
  1. Jill M. Siegfried
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  2. Karen G. Nelson
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  3. Jane L. Martin
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  4. David G. Kaufman
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Additional information

J. M. S. was recipient of National Research Service Award CA09156 (National Cancer Institute); K. G. N. was recipient of National Research Service Award ES07017 (National Institute of Environmental Health Sciences); and D. G. K. was recipient of Research Career Development Award CA00431 from the National Cancer Institute, Bethesda, MD. Supported by Grant CA 31733 from the National Cancer Institute, Bethesda, MD.

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Siegfried, J.M., Nelson, K.G., Martin, J.L. et al. Histochemical identification of cultured cells from human endometrium. In Vitro 20, 25–32 (1984). https://doi.org/10.1007/BF02633328

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  • DOI: https://doi.org/10.1007/BF02633328

Key words

  • endometrium
  • human cell culture
  • histochemistry