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The chemical and biological assay of essential fatty acids

  • Technical
  • Symposium on Methodology of Fats and Oils Conducted by the American Oil Chemists' Society at Its 53rd Annual Meeting, New Orleans, Louisiana May 8–9, 1962
  • Published:
Journal of the American Oil Chemists Society

Abstract

After defining and developing the background of essential fatty acids, the various chemical, biological and physical methods which have been used for their assay will be reviewed. Specific recommendations will be made regarding techniques in each method category for natural and processed fats. Special attention will be given to the distinction between the essential fatty acids and the other polynnsaturated acids.

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References

  1. Ault, W. C., and J. B. Brown, J. Biol. Chem.,107, 615 (1939).

    Google Scholar 

  2. Beadle, B. W., and H. R. Kraybill, J. Am. Chem. Soc.,66, 1232 (1944).

    Article  CAS  Google Scholar 

  3. Beerthuis, R. K., G. Dijkstra, J. G. Keppler, and J. H. Recourt, Ann. New York Acad. Sci.,72, 616 (1959).

    Article  CAS  Google Scholar 

  4. Brown, J. B., and J. Frankel, J. Am. Chem. Soc.,60, 54 (1938).

    Article  CAS  Google Scholar 

  5. Burr, G. O., and M. M. Burr, J. Biol. Chem.,82, 345 (1929).

    CAS  Google Scholar 

  6. Cason, J., and P. Tavs,Ibid., 234, 1401 (1959).

    PubMed  CAS  Google Scholar 

  7. Craig, B. M., and N. L. Murty, JAOCS,36, 549 (1959).

    Google Scholar 

  8. Evans, H. M., S. Lepkovsky, and E. A. Murphy, J. Biol. Chem.,106, 441 (1934).

    CAS  Google Scholar 

  9. Farquhar, J. W., W. Insull, Jr., P. Rosen, W. Stoffel, and E. H. Ahrens, Jr., Nutrition Reviews, Vol. 17, No. 8, Part II, (1959).

  10. Fenton, A. J., Jr., and R. O. Crisler JAOCS,36, 620 (1959).

    CAS  Google Scholar 

  11. Greenberg, S. M., C. E. Calbert, E. E. Savage, and H. J. Deuel, Jr., J. Nutrition,41, 473 (1950).

    CAS  Google Scholar 

  12. Hammond, E. G., and W. O. Lundberg, JAOCS,30, 433 (1953).

    CAS  Google Scholar 

  13. Herb, S. F., P. Magidman, and R. W. Riemenschneider,Ibid. 37, 127 (1960).

    CAS  Google Scholar 

  14. Herb, S. R., and R. W. Riemenschneider, Anal. Chem.,25, 953 (1953).

    Article  CAS  Google Scholar 

  15. Herb, S. F., and R. W. Riemenschneider, JAOCS,29, 456 (1952).

    CAS  Google Scholar 

  16. Holman, R. T., and P. R. Edmondson, Anal. Chem.,28, 1533 (1956).

    Article  CAS  Google Scholar 

  17. Holman, R. T., and S. Ener, J. Nutrition,53, 461 (1954).

    CAS  Google Scholar 

  18. Holman, R. T., S. Ener, and P. R. Edmondson, Arch. Biochem. Biophys.,80, 72 (1959).

    Article  CAS  Google Scholar 

  19. Holman, R. T., and S. I. Greenberg,Ibid., 49, 49 (1954).

    Article  PubMed  CAS  Google Scholar 

  20. Inouye, Y., M. Noda, and O. Hirayama, JAOCS,32, 132 (1955).

    Google Scholar 

  21. James, A. T., Fette u. Seifen,59, 73 (1957).

    Article  CAS  Google Scholar 

  22. James, A. T., J. Chrom.,2, 552 (1959).

    Article  CAS  Google Scholar 

  23. James, A. T., and J. Webb, Biochem. J.,66, 515 (1957).

    PubMed  CAS  Google Scholar 

  24. Kaufmann, H. P., Ber. pharm. Ges.,33, 139 (1923).

    CAS  Google Scholar 

  25. Kaufmann, H. P., Z. Untersuch. Lebensm,51, 15 (1926).

    Article  CAS  Google Scholar 

  26. Kaufmann, H. P., and E. Mohr, Fette u. Seifen,60, 165 (1958).

    Article  CAS  Google Scholar 

  27. Kaufmann, H. P., and H. Schnurbusch,Ibid., 60, 1046 (1958).

    Article  CAS  Google Scholar 

  28. Kaufmann, H. P., and W. H. Nitsch,Ibid., 58, 234 (1956).

    Article  CAS  Google Scholar 

  29. Kaye, W., Applied Spectroscopy,15, 89 (1961).

    Article  CAS  Google Scholar 

  30. Landowne, R. A., and S. R. Lipsky, Biochim. et Biophys. Acta,46, 1 (1961).

    Article  CAS  Google Scholar 

  31. Litchfield, C., JAOCS,40, 2- (1963).

    Google Scholar 

  32. MacGee, J., Anal. Chem.,31, 298 (1959).

    Article  CAS  Google Scholar 

  33. Mangold, H. K., and D. C. Malins, JAOCS,37, 383 (1960).

    CAS  Google Scholar 

  34. Mangold, H. K., and R. Kammereck, Chem. Ind. (London) No.27, 1032 (1961).

    Google Scholar 

  35. Mead, J.F., J. Biol. Chem.,227, 1025 (1957).

    PubMed  CAS  Google Scholar 

  36. Mead, J. F., and D. R. Howton, “Interconversions of the Unsaturated Fatty Acids” in H. M. Sinclair, ed., Essential Fatty Acids, Academic Press Inc., New York, 1958, p. 65.

    Google Scholar 

  37. Mitchell, J. H., H. R. Kraybill and F. P. Zscheile, Ind. Eng. Chem., anal. ed.,15, 1 (1943).

    Article  CAS  Google Scholar 

  38. Moore, T., Biochem. J.,31, 138 (1937).

    PubMed  CAS  Google Scholar 

  39. Morris, L. J., R. T. Holman, and K. Fontell, JAOCS,37, 323 (1960).

    CAS  Google Scholar 

  40. Morris, L. J., R. T. Holman, and K. Fontell, J. Lipid Res.,1, 421 (1960).

    Google Scholar 

  41. Morris, L. J., R. T. Holman, and K. FontellIbid. 2, 77 (1961).

    CAS  Google Scholar 

  42. AOCS Official and Tentative Method Cd 2-38. Additions and Revisions 1947 through 1958.

  43. AOCS Official and Tentative Method Cd 7-58. Additions and Revisions 1947 through 1958.

  44. Orr, C. H., and J. E. Callen, J. Am. Chem. Soc.,80, 248 (1958).

    Article  Google Scholar 

  45. Ory, R. L., W. G. Bickford, and J. W. Dieckert, Anal. Chem.,31, 1447 (1959).

    Article  CAS  Google Scholar 

  46. Perilä, O., Acta. Chem. Scand.,10, 143 (1956).

    Article  Google Scholar 

  47. Petrowitz, H. J., Angew. Chem.,72, 921 (1960).

    CAS  Google Scholar 

  48. Pitt, G. A. J., and R. A. Morton, “Ultra-violet Spectrophotometry of Fatty Acids,” in R. T. Holman, W. O. Lundberg, and T. Malkin, ed., Progress in the Chemistry of Fats and Other Lipids, Vol. 4 Pergammon Press, New York, 1957, p. 228.

    Google Scholar 

  49. Popjak, G., and A. Tietz, Biochem. J.56, 46 (1954).

    PubMed  CAS  Google Scholar 

  50. Ramsey, L. L., and W. I. Patterson, J. Assoc. Offic. Agr. Chemists,31, 139 (1948).

    CAS  Google Scholar 

  51. Reiser, R., N.L. Murty, and H. Rakoff, J. Lipid Res.,3, 56 (1962).

    CAS  Google Scholar 

  52. Riemenschneider, R. W., S. F. Herb, and P. L. Nichols, Jr., JAOCS,26, 371 (1949).

    CAS  Google Scholar 

  53. Rusoff, I. I., J. R. Platt, H. B. Klevens, and G. O. Burr, J. Am. Chem. Soc.,67, 673 (1945).

    Article  CAS  Google Scholar 

  54. Savary, P., and P. Desneulle, Bull. soc. chim., France,20, 939 (1953).

    Google Scholar 

  55. Schlenk, H., J. L. Gellerman, J. A. Tillotson, and H. K. Mangold, JAOCS,34, 377 (1957).

    CAS  Google Scholar 

  56. Schmidt, G., Naturwissenschaften,45, 41 (1958).

    Article  CAS  Google Scholar 

  57. Seher, A., Fette u. Seifen,61, 55 (1959).

    Google Scholar 

  58. Shinowara, G. Y., and J. B. Brown, J. Am. Chem. Soc.,60, 2734 (1938).

    Article  CAS  Google Scholar 

  59. Steinberg, G., W. H. Slaton, Jr., D. R. Howton, and J. F. Mead, J. Biol. Chem.,224, 841 (1957).

    PubMed  CAS  Google Scholar 

  60. Stoffel, W., and E. H. Ahrens, Jr., J. Lipid Research,1, 139 (1960).

    CAS  Google Scholar 

  61. Stoffel, W., W. Insull, Jr., and E. H. Alrens, Jr., Proc. Soc. Expt. Biol. Med.,99, 238 (1958).

    CAS  Google Scholar 

  62. Storey, W. H., Jr., JAOCS,37, 676 (1960).

    CAS  Google Scholar 

  63. Sulser, H., Mitt. Gebiete Lebensmitt. u. Hyg.,50, 275 (1959).

    CAS  Google Scholar 

  64. Swartwout, J. R., J. W. Dieckert, O. N. Miller, and J. G. Hamilton, J. Lipid Research1, 281 (1960).

    CAS  Google Scholar 

  65. Thomasson, H., Intern. Rev. Vitamin Research,25, 62 (1953).

    CAS  Google Scholar 

  66. Thomasson, H., Rivista Ital. delle Sostanze Grasse38, 541 (1961).

    CAS  Google Scholar 

  67. van Beers, G. J., S. Sparreboom, and J. G. Keppler, “The determination ofcis, cis-linolic acid in the presence of its geometric isomers,” in H. M. Sinclair, ed., Essential Fatty Acids, Academic Press Inc., New York, 1958, p. 16.

    Google Scholar 

  68. Vanderzant, E. S., D. Kerur, and R. Reiser, J. Econ. Entomol.,50, 606 (1957).

    CAS  Google Scholar 

  69. Wheeler, D. H., “Infrared Absorption Spectroscopy in Fats and Oil,” in R. T. Holman, W. O. Lundberg, and T. Malkin, ed., Progress in the Chemistry of Fats and Other Lipds, Vol. 2, Academic Press Inc., New York, 1954, p. 268.

    Google Scholar 

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Outlined analysis of paper soon to appear in Vol. I of a series, “Analysis and Characterization of Oils, Fats and Fat Products,” H. Boekenoogen, editor, John Wiley & Sons, Ltd., London.

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Williams, M.C., Reiser, R. The chemical and biological assay of essential fatty acids. J Am Oil Chem Soc 40, 237–241 (1963). https://doi.org/10.1007/BF02632668

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  • DOI: https://doi.org/10.1007/BF02632668

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