Skip to main content
Springer Nature Link
Log in

Methanol-precipitated, detergent-treated newcastle disease virus as an agar-gel precipitation test antigen

Utilisation D’un Virus De La Maladie De Newcastle Traite Par Detergent Et Precipite Au Methanol Pour La Preparation D’un Antigene Dans Un Test De Precipitation Sur Agarose

Utilizacion De Virus De La Enfermedad De Newcastle Precipitado Con Metanol Y Tratado Con Detergente Como Antigeno En Un Test De Precipitacion En Agar Gel

  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Summary

Methanol-precipitated, detergent-treated (Triton X-100) Newcastle disease virus (NDV) was found suitable as an agar gel precipitation test (AGPT) antigen. One hundred and twenty sera were tested by the haemagglutination-inhibition (HI) test and AGPT against NDV. There was a very significant increase in the proportion of AGPT positive samples with increase in HI titre. Hence AGPT can be recommended as a field based test for seromonitoring following vaccinations against ND where laboratory facilities are inadequate.

Résumé

Un virus de la maladie de Newcastle, traité par un détergent (Triton X-100 et précipité au méthanol fut reconnu utilisable comme antigène pour un test de précipitation sur agarose (AGPT). 120 serums furent testés par test d’inhibition hemo-agglutinant et par AGPT contre le virus de la maladie de Newcastle. Il y eut une augmentation significante dans la proportion d’échantillons positifs par AGPT avec l’augmentation du titre Hi. Désormais l’AGPT peut être recommandé comme un test de terrain pour le serodiagnostic après vaccination contre la maladie de Newcastle lorsque les facilités de laboratoire ne sont pas adéquates.

Resumen

Virus de la enfermedad de Newcastle (NDV) precipitados con metanol y tratados con detergente (Triton X-100) resultaron ser adecuados como antígenos para un test de precipitación en agar gel (AGPT). Se realizaron AGPT y tests de inhibición de la hemoaglutinación en 120 muestras de suero. Se encontró un aumento muy significativo en la proporción de muestra positivas según AGPT conforme aumentaba el título de anticuerpos determinado según HI. Por tanto, cuando las instalaciones de laboratorio no son adecuadas, AGPT puede ser recomendado como test de campo para seromonitorización después de vacunar frente a la enfermedad de Newcastle.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  • Allan, W. H. &Gough, R. E. (1974). A standard haemagglutination inhibition test for Newcastle disease 1. A comparison of macro and micro methods.Veterinary Record,95, 120–123.

    Article  PubMed  CAS  Google Scholar 

  • Fenner, F., McAuslan, B. R., Mims, C. A. Sambrok, J. &White, D. O. (1974).The Biology of Animal Viruses. Academic press, New York, USA, pp 65–67.

    Google Scholar 

  • Gelb, J. Jr &Cianci, C. G. (1987). Detergent treated Newcastle disease virus as gel precipitation test antigen.Poultry Science,66, 845–853.

    PubMed  Google Scholar 

  • Lupton, L. W. &Reed, D. E. (1980). Evaluation of experimental subunit vaccine for infectious bovine rhinotracheitis.American Journal of Veterinary Research,41, 383–390.

    PubMed  CAS  Google Scholar 

  • Makino, S., Reynolds, J. A. &Tanford, C. (1973). The binding of deoxycholate and triton X-100 to proteins.Journal of Biological Chemistry,248, 4926–4932.

    PubMed  CAS  Google Scholar 

  • Miers, L. A., Bankowski, R. A. &Zee, Y. C. (1983). Optimizing the enzyme-linked immunosorbent assay for evaluating immunity of chickens to Newcastle disease.Avian Diseases,27, 1112–1125.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, 600 051, Madras, India

    Parimal Roy, A. Koteeswaran & A. T. Venugopalan

Authors
  1. Parimal Roy
    View author publications

    Search author on:PubMed Google Scholar

  2. A. Koteeswaran
    View author publications

    Search author on:PubMed Google Scholar

  3. A. T. Venugopalan
    View author publications

    Search author on:PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Roy, P., Koteeswaran, A. & Venugopalan, A.T. Methanol-precipitated, detergent-treated newcastle disease virus as an agar-gel precipitation test antigen. Trop Anim Health Prod 29, 25–28 (1997). https://doi.org/10.1007/BF02632342

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02632342

Keywords