Carbendazim as an alternative plant growth regulator in tissue culture systems

  • P. C. Debergh
  • G. de Coster
  • W. Steurbaut


Carbendazim is the fungitoxic ingredient of different fungicides. In our experiments it was used as a supplement to stage II (multiplication) media for the micropropagation ofCordyline terminalis andPrunus avium. The product can be autoclaved without any loss of activity and there is no degradation of the product over a normal culture period of 32 days. WithC. terminalis andP. avium no phytotoxic effect was revealed up to 160µg/ml. ForC. terminalis shoot height was reduced and the number of shoots smaller than 15 mm increased significantly.

Key words

novel PGRs Derosal L500 (BASF) shoot multiplication stage II cultures fungitoxic chemicals Cordyline terminalis Prunus avium 


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  1. 1.
    Brown, D. M.; Groom, C. L.; Cvitanik, M., et al. Effects of fungicides and bactericides on orchid seed germination and shoot tip culturesin vitro. Plant Cell Tissue Organ Cult. 1:165–180; 1982.CrossRefGoogle Scholar
  2. 2.
    Evaldsson, I. E.; Welander, N. T. The effects of medium composition onin vitro propagation andin vivo growth ofCordyline terminalis cv Atoom. J. Hortic. Sci. 60:525–530; 1985.Google Scholar
  3. 3.
    Jones, O. P.; Hopgood, M. E. The successful propagationin vitro of two rootstocks ofPrunus: the plum rootstock ‘Pixy’ (P. insititia) and the cherry ‘F12/1’ (P. avium). J. Hortic. Sci. 54:63–66; 1979.Google Scholar
  4. 4.
    Letham, D. S. A new cytokinin bioassay and the naturally occurring cytokinin complex. In: Wightman, F.; Setterfield, G., eds. Biochemistry and physiology of plant growth substances. Ottawa: The Runge Press; 1968:919–931.Google Scholar
  5. 5.
    Maene, L.; Debergh, P.Cordyline andDracaena. In: Ammirato, P. V.; Evans, D. R.; Sharp, W. R., et al., eds. Handbook of plant cell culture, vol. 5. New York: McGraw-Hill Publishing Cy; 1990:337–351.Google Scholar
  6. 6.
    Maxwell, W. A.; Brody, G. Antifungal activity of selected benzimidazole compounds. Appl. Microbiol. 21:944–945; 1971.PubMedGoogle Scholar
  7. 7.
    Miller, C. O.; Naturally occurring cytokinins. In: Wightman, F.; Setterfield, G., eds. Biochemistry and physiology of plant growth substances. Ottawa: The Runge Press; 1968:33–45.Google Scholar
  8. 8.
    Person, C.; Samborski, D. J.; Forsyth, F. R. Effect of benzimidazole on detached wheat leaves. Nature 180:1294–1295; 1957.CrossRefGoogle Scholar
  9. 9.
    Samborski, D. J.; Forsyth, F. R.; Person, C. Metabolic changes in detached wheat leaves floated on benzimidazole and the effect of these changes on rust reaction. Can. J. Bot. 36:591–601; 1958.CrossRefGoogle Scholar
  10. 10.
    Schruft, G. Die Wirkung desBotrytis-Präparates Benomyl inAmaranthus cytokinin test. Wein-Wissenschaft 25:329–333; 1970.Google Scholar
  11. 11.
    Shields, R.; Robinson, S. J.; Anslow, P. A. Use of fungicides in plant tissue culture. Plant Cell Rep. 3:33–36; 1984.CrossRefGoogle Scholar
  12. 12.
    Skene, K. G. M. Cytokinin-like properties of the systemic fungicide benomyl. J. Hortic. Sci. 47:179–182; 1972.Google Scholar
  13. 13.
    Steurbaut, W.; De Jonckheere, W.; Kips, R. H. Fate of benomyl on early autumn and spring glasshouse lettuce. Med. Fac. Landbouww. Rijksuniv. Gent 38:917–928; 1973.Google Scholar
  14. 14.
    Thurston, K. C.; Spencer, S. J.; Arditti, J. Phytotoxicity of fungicides and bactericides in orchid culture media. Am. J. Bot. 66:825–835; 1979.CrossRefGoogle Scholar
  15. 15.
    Tripathi, R. K.; Ram, S. Induction of growth and differentiation of carrot callus cultures by carbendazim and benzimidazole. Ind. J. Exp. Biol. 20:674–677; 1982.Google Scholar
  16. 16.
    Yang, H. J. Effect of benomyl onAsparagus officinalis L. Shoot and root development in culture media. HortScience 11:473–474; 1976.Google Scholar

Copyright information

© Tissue Culture Association 1993

Authors and Affiliations

  • P. C. Debergh
  • G. de Coster
  • W. Steurbaut
    • 1
  1. 1.Laboratory of Horticulture and Laboratory of PhytopharmacyUniversity of GentGentBelgium

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