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Isolated microspore culture of wheat (Triticum aestivum L.) in a defined media I. Effects of pretreatment, isolation methods, and hormones

  • Developmental Biology/Morphogenesis
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Abstract

Significant improvements were achieved in the production of haploid and doubled haploid plants from isolated microspore culture of wheat c.v. Chris on a defined media. Procedures found to be of benefit included: A 7-day pretreatment of anthers in 0.4M mannitol plus the macronutrients from FHG medium; the inclusion of 4.5 mg/liter abscisic acid in the pretreatment solution; the isolation of microspores from pretreated anthers by vortexing; and the use of phenylacetic acid (PAA) as the auxin source in MS medium. The best response was achieved with 4.0 mg/liter PAA in MS medium containing 90 g/liter maltose as the sugar source. Under these conditions, 68% of viable microspores underwent division, and an average of 93 embryos and 92 green plants were regenerated per 100 anthers used. The root-tip chromosome number and the fertility of 114 regenerating green plants revealed that 75% were completely fertile spontaneously doubled haploids.

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References

  • Cho, U. H.; Kasha, K. J. Relationship of senescence to androgenesis in barley (Hordeum vulgare L. cv. Klages). J. Plant Physiol. 139:299–302; 1992.

    CAS  Google Scholar 

  • Chu, C. C.; Hill, R. D.; Brule-Babel, A. L. High frequency of pollen embryoid formation and plant regeneration inTriticum aestivum L. on monosaccharide containing media. Plant Sci. 66:255–262; 1990.

    Article  CAS  Google Scholar 

  • Datta, S. K.; Wenzel G. Isolated microspore derived plant formation via embryogenesis inTriticum aestivum L. Plant Sci. 48:49–54; 1987.

    Article  Google Scholar 

  • Foroughi-Wehr, B.; Mix, G. In vitro response ofHordeum vulgare L. anthers cultures from plants grown under different environment. Environ. Exp. Bot. 19:303–309; 1979.

    Article  Google Scholar 

  • Hoekstra, S.; Zijderveld, M. H. V.; Louwerse, J. D., et al. Anther and microspore culture ofHordeum vulgare L. cv. Igri. Plant Sci. 86:89–96; 1992.

    Article  CAS  Google Scholar 

  • Huang, B.; Sunderland, N. Temperature-stress pretreatment in barley anther culture. Ann. Bot. 49:77–88; 1982.

    Google Scholar 

  • Hunter, C. P. Plant regeneration from microspores of barley (Hordeum vulgare). England: Wye College, Univ. London; 1988. Thesis.

    Google Scholar 

  • Johnsson, L.; Eriksson, T. Induced embryo information in anther cultures of severalAnemone species. Physiol. Plant. 40:172–172; 1977.

    Article  Google Scholar 

  • Kasha, K. J.; Ziauddin, A.; Cho, U. H. Haploids in cereal improvement: anther and microspore culture. In: Gustafson, J. P., ed. Gene manipulation in plant improvement II. New York; Plenum Press; 1990:213–235.

    Google Scholar 

  • Kasha, K. J.; Cho, U. H.; Ziauddin, A. Application of microspore cultures. In: Munck L. ed. Barley genetics VI. Munksgaard Int. Publishers. Copenhagen, Denmark: 1992:793–806.

    Google Scholar 

  • Klein, T. M.; Wolf, E. D.; Wu, R.; Sanford, J. C. High velocity microprojectiles for delivering nucleic acids into living cells. Nature 327:70–73; 1987.

    Article  CAS  Google Scholar 

  • Köhler, F.; Wenzel, G. Regeneration of isolated barley microspores in conditioned media and trials to characterize the responsible factor. J. Plant Physiol. 121:181–191; 1985.

    Google Scholar 

  • Leuba, V.; LeTourneau, D., Oliver, D. Stability of Phenylacetic acid in liquid media. J. Plant Growth Regul. 8:163–165; 1989.

    Article  Google Scholar 

  • Mejza, S. J.; Morgant, V.; DiBona, D. E.; Wong, J. R. Plants regeneration from isolated microspores ofTriticum aestivum L. Plant Cell Rep. 12:149–153; 1993.

    Article  Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  • Olsen, L. Isolation and cultivation of embryogenic microspores from barley (Hordeum vulgare L.) Carlsberg Res. Comm. 52:393–404; 1987.

    Article  CAS  Google Scholar 

  • Petroski, R. Cytological changes during anther culture of barley (Hordeum vulgare L.). Ontario, Canada: Univ. Guelph, 1991. Thesis.

    Google Scholar 

  • Sanford, J. C.; Klein, T. M.; Wolf, E. D.; Allen, N. Delivery of substances into cells and tissues using a particle bombardment process. Part. Sci. Tech. 5:27–37; 1987.

    CAS  Google Scholar 

  • Torrizo, L. B.; Zapata, F. J. Anther culture in rice: IV. The effects of abscisic acid on plant regeneration. Plant Cell Rep. 5:136–139; 1986.

    Article  CAS  Google Scholar 

  • Tuvesson, I. K. D.; Öhlund, R. C. V. Plant regeneration through culture of isolated microspores ofTriticum aestivum L. Plant Cell Tissue Organ Cult. 34:163–167; 1993.

    Article  Google Scholar 

  • Wei, Z. M. Pollen Callus culture inTriticum aestivum L. Theor. Appl. Genet. 63:71–73; 1982.

    Article  Google Scholar 

  • Ziauddin, A.; Marsolais, A.; Simion, E.; Kasha, K. J. Improved plant regeneration from wheat anther and barley microspore culture using phenylacetic acid (PAA). Plant Cell Rep. 11:489–498; 1992.

    Article  CAS  Google Scholar 

  • Ziauddin, A.; Simion, E.; Kasha, K. J. Improved plant regeneration from shed microspore culture in barley (Hordeum vulgare L.) cv. Igri. Plant Cell Rep. 9:69–72; 1990.

    Article  Google Scholar 

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Hu, T.C., Ziauddin, A., Simion, E. et al. Isolated microspore culture of wheat (Triticum aestivum L.) in a defined media I. Effects of pretreatment, isolation methods, and hormones. In Vitro Cell Dev Biol - Plant 31, 79–83 (1995). https://doi.org/10.1007/BF02632241

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  • DOI: https://doi.org/10.1007/BF02632241

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