Summary
Cultures ofDigitalis obscura L. were established from axillary buds of mature plants or leaves of seedlings obtained under aseptic conditions. Explants were cultured on Murashige and Skoog medium containing benzyladenine and/or naphthaleneacetic acid. Shoot proliferation from axillary buds was not affected by seasonal fluctuations in the stock plants and increased relative to the cytokinin concentration, but auxin reduced the multiplication rate. Differentiation of somatic embryos and adventitious buds from cultured leaves required naphthaleneacetic acid alone or combined with benzyladenine, respectively. Cardenolide pattern and content of the regenerated plants were determined by high performance liquid chromatography and radioimmunoassay, respectively. Several cardenolides of series A and C were identified in the regenerants; no significant differences were found in the cardenolide patterns. Digoxigenin derivatives were found in all clonally propagated plants, but the amount of these glycosides was much higher in those obtained from axillary buds. This is the first report on micropropagation ofD. obscura from mature plants.
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The financial support of CICYT, Madrid, Spain (project no. PB89-0419) is gratefully acknowledged.
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Vela, S., Gavidia, I., Pérez-Bermúdez, P. et al. Micropropagation of juvenile and adultDigitalis obscura and cardenolide content of clonally propagated plants. In Vitro Cell Dev Biol - Plant 27, 143–146 (1991). https://doi.org/10.1007/BF02632198
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DOI: https://doi.org/10.1007/BF02632198