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Abnormal growth of habituated sugarbeet callus and cell suspensions

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Summary

Habituated sugarbeet callus and cell suspension cultures derived therefrom, compared to hormone-dependent normal cultures, exhibit a shorter linear growth phase, although they divide actively. Microscopic observations indicate deficiencies in cell expansion and absence of cell differentiation. Cell expansion apparently is interrupted by a cell “budding” process. Some of the cells seem to be empty due to ballooning out of the protoplasm and the bursting of the cell membrane by defective cell wall development. A low amount of cellulose was confirmed by microspectrophotometric estimation. Such cultures exhibit all the characteristics of vitrified tissue.

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Authors and Affiliations

  1. Laboratoire de Physiologie et Biochimie végétales, Université de Caen, F-14032, Caen Cedex, France

    Daniel Hagege

  2. Hormonologie fondamentale et appliquée, Institut de Botanique B22, Université de Liège, Sart Tilman, B-4000, Liège, Belgique

    Claire Kevers & Thomas Gaspar

  3. Plant Physiology Research Group, Department of Biological Sciences, University of Calgary, T2N 1N4, Alberta, Canada

    Trevor A. Thorpe

Authors
  1. Daniel Hagege
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  2. Claire Kevers
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  3. Thomas Gaspar
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  4. Trevor A. Thorpe
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Hagege, D., Kevers, C., Gaspar, T. et al. Abnormal growth of habituated sugarbeet callus and cell suspensions. In Vitro Cell Dev Biol - Plant 27, 112–116 (1991). https://doi.org/10.1007/BF02632193

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  • DOI: https://doi.org/10.1007/BF02632193

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