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Expression ofβ-galactosidase and luciferase in insect cell lines infected with a recombinant AcMNPV

  • Session-In-Depth: Insect Host-Pathogen And Parasite Interaction In Vitro
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Summary

An AcMNPV recombinant (Ac-gal-luc) carrying theβ-galactosidase and luciferase genes under the control of the p10 and polyhedrin promoters, respectively, was used to study expression in nine insect cell lines. All AcMNPV-permissive cell lines expressed both reporter genes with the coleopteran cell line,Anthonomus grandis (AGE), producing the highest concentrations ofβ-galactosidase (5.0×106 pg/ml) and luciferase (2.67×103 pg/ml). Both enzymes were detected as early as 12 h postinoculation in lysate samples of the AGE cell line.Helicoverpa armigera (HA), a nonpermissive cell line, expressedβ-galactosidase at 72 h postinoculation at a concentration of 3.5×103 pg/ml. However, expression of luciferase was not detected. Expression of luciferase andβ-galactosidase was also not detected in the nonpermissiveHelicoverpa zea (HZ) cell line.

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  1. ARS Biological Control of Insects Research Laboratory, USDA, 65205, Columbia, Missouri

    Arthur H. McIntosh & James J. Grasela

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  1. Arthur H. McIntosh
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  2. James J. Grasela
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McIntosh, A.H., Grasela, J.J. Expression ofβ-galactosidase and luciferase in insect cell lines infected with a recombinant AcMNPV. In Vitro Cell Dev Biol - Animal 30, 275–278 (1994). https://doi.org/10.1007/BF02632051

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  • DOI: https://doi.org/10.1007/BF02632051

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