Summary
In order to identify the physiological and biochemical events leading to the negative effects of the presence of sucrose in culture medium on the photosynthetic capacity of plantlets cultivated in vitro, time course in photosynthesis, metabolite pool sizes, and ribulose-1,5-bisphosphate carboxylase (EC 4.1.1.39) activity were investigated in strawberry (Fragaria x ananassa Duch. cv. Kent) plantlets following their transfer to medium with or without sucrose. When the plantlets grown in medium without sucrose were transferred to a similar medium with 30 g liter−1 sucrose, their net photosynthesis decreased and their level of phosphorylated compounds increased with time. In addition, initial catalytic turnover, total catalytic turnover, and the activation state of ribulose-1,5-bisphosphate carboxylase decreased in these plantlets. Conversely, when the plantlets grown in medium with 30 g liter−1 sucrose were transferred to a similar medium without sucrose, their net photosynthesis slowly increased with time and their level of phosphorylated compounds slowly decreased. A slow increase with time of initial catalytic turnover, total catalytic turnover, and the activation state of ribulose-1,5-bisphosphate carboxylase was also observed in these plantlets. The results of the present paper suggest that the reduced photosynthetic capacity of strawberry plantlets cultivated in vitro in the presence of sucrose is the consequence of a reduction in the efficiency of ribulose-1,5-bisphosphate carboxylase/oxygenase due to its deactivation and the possible presence of putative inhibitors of carboxylation sites.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Avelange, M. H.; Sarrey, F.; Rébillé, F. Effects of glucose feeding on respiration and photosynthesis in photoautotrophicDianthus caryophyllus cells. Plant Physiol. 94:1157–1162; 1990.
Azcon-Bieto, J. The control of photosynthesis gas exchange by assimilate accumulation in wheat. In: Marcelle, R.; Clijsters, H.; Van Poucke, M., eds. Biological control of photosynthesis. Dordrecht, The Netherlands: Martinus Nijhoff Publishers; 1986:231–240.
Badger, M. R.; Sharkey, T. D.; Von Caemmerer, S. The relation-ship between steady-state gas exchange of bean leaves and the levels of carbonreduction-cycle intermediates. Planta 160:305–313; 1984.
Bradford, M. M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248–254; 1976.
Brainerd, K. E.; Fuchigami, L. H.; Kwiatkowski, S., et al. Leaf anatomy and water stress of aseptically cultured “Pixy” plum grown under different environments. HortScience 16:173–175; 1981.
Capellades, M.; Lemeur, R.; Debergh, P. Effects of sucrose on starch accumulation and rate of photosynthesis in Rosa cultured in vitro. Plant Cell Tissue Organ Cult. 25:21–26; 1991.
Collatz, G. J.; Badger, M. R.; Smith, C., et al. A radioimmune assay for RuP2 carboxylase protein. Carnegie Institution. Washington Yearbook 78:171–175; 1979.
Desjardins, Y.; Gosselin, A.; Yelle, S. Acclimatization of ex vitro strawberry plantlets in CO2-enriched environments and supplementary lighting J. Am. Soc. Hortic. Sci. 112:846–851; 1987.
Foyer, C. H. Feedback inhibition of photosynthesis through source-sink regulation in leaves. Plant Physiol. Biochem. 26:483–492; 1988.
Giersch, C.; Heber, H.; Kaiser, G., et al. Intracellular metabolite gradients and flow of carbon during photosynthesis of leaf protoplasts. Arch. Biochem. Biophys. 205:246–259; 1980.
Grout, B. W. W.; Donkin, M. E. Photosynthetic activity of cauliflower meristem culturesin vitro and at transplanting into soil. Acta Hortic. 212:323–327; 1987.
Hagimori, M.; Matsumoto, T.; Mikami, Y. Photoautotrophic culture of undifferentiated cells and shoot-growing cultures ofDigitalis purpurea L. Plant Cell Physiol. 25:1099–1102; 1984.
Hdider, C.; Desjardins, Y. Prevention of shoot vitrification of strawberry micropropagated shoots proliferated on liquid media by new antivitrifying agents. Can. J. Plant Sci. 73:231–235; 1993.
Hdider, C.; Desjardins, Y. Effects of sucrose on photosynthesis and phosphoenolpyruvate carboxylase activity ofin vitro cultured strawberry plantlets. Plant Cell Tissue Organ Cult. 36:27–33; 1994.
Kozai, T.; Sekimoto, K. Effects of the number of air changes per hour of the closed vessel and the photosynthetic photon flux on the carbon dioxide concentration inside the vessel and the growth of strawberry plantletsin vitro. Environ. Control Biol. 26:21–29; 1988.
Krapp, A.; Stitt, M. Influence of high carbohydrate content on the activity of plastidic and cytosolic isozyme pairs in photosynthetic tissues. Plant Cell Environ. 17:861–866; 1994.
Langford, P. J.; Wainwright, H. Effects of sucrose concentration on the photosynthetic ability of rose shootsin vitro. Ann. Bot. 60:633–640; 1987.
Leegood, R. C.; Von Caemmerer, S. The relationships between content of photosynthetic metabolites and the rate of photosynthetic carbon assimilation in leaves ofAmaranthus edulis. Planta 174:253–262; 1988.
Lees, R. P.; Evans, E. H.; Nicholas, J. R. Photosynthesis in Clematis, “the president”, during growthin vitro and subsequentin vivo acclimatization. J. Exp. Bot. 42:605–610; 1991.
Miziorko, H. M.; Lorimer, G. H. Ribulose-1,5-bisphosphate carboxylase-oxygenase. Annu. Rev. Biochem. 52:507–535; 1983.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:473–497; 1962.
Pospisilova, J.; Solarova, J.; Catsky, J. Photosynthetic responses to stresses duringin vitro cultivation. Photosynthetica 26:3–18; 1992.
Potris, A. R., Jr. Regulation of Ribulose 1,5-biphosphate carboxylase/oxygenase activity. Annu. Rev. Plant Physiol. Plant Mol. Biol. 43:415–437; 1992.
Sage, R. F. A model describing the regulation of ribulose-1,5-bisphosphate carboxylase, electron transport, and triose phosphate use in response to light intensity and CO2 in C3 plants. Plant Physiol. 94:1728–1734; 1990.
Seemann, J. R. Mechanisms for the regulation of CO2 fixation by ribulose-1,5-bisphosphate carboxylase. In: Marcelle, R.; Clijsters, H.; Van Poucke, M., eds. Biological control of photosynthesis. Dordrecht, The Netherlands: Martinus Nijhoff Publishers; 1986:71–82.
Seemann, J. R.; Sharkey, T. D. Salinity and nitrogen effects on photosynthesis, ribulose-1,5-bisphosphate carboxylase and metabolite pool sizes inPhaseolus vulgaris L. Plant Physiol. 82:555–560; 1986.
Sestak, Z.; Catsky, J.; Jarvis, P. G. Plant photosynthetic production: manual of methods. The Hague, The Netherlands: Dr. W. Junk N. V. Publishers; 1971:519.
Sharkey, T. D. Feedback limitation of photosynthesis and the physiological role of ribulose bisphosphate carbamylation. Bot. Mag. Tokyo (Special Issue) 2:87–105; 1990.
Sharkey, T. D.; Seemann, J. R.; Berry, J. A. Regulation of ribulose-1,5-bisphosphate carboxylase in response to changing partial pressure of O2 and light inPhaseolus vulgaris. Plant Physiol. 81:788–791; 1986.
Sharkey, T. D.; Vanderveer, P. J. Stromal phosphate concentration is low during feedback limited photosynthesis. Plant Physiol. 91:679–684; 1989.
Short, K. C.; Warburton, J.; Roberts, A. V.In vitro hardening of cultured cauliflower and Chrysanthemum plantlets to humidity. Acta Hortic. 212:329–334; 1987.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hdider, C., Desjardins, Y. Reduction of ribulose-1,5-bisphosphate carboxylase/oxygenase efficiency by the presence of sucrose during the tissue culture of strawberry plantlets. In Vitro Cell Dev Biol - Plant 31, 165–170 (1995). https://doi.org/10.1007/BF02632014
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02632014