Summary
MicropropagatedSpathiphyllum “Petite” plantlets were acclimatized at low- or high-light intensities [photosynthetic photon flux density (PPFD) 100 or 300 µmol·m−2·s−1]. During the first days chlorophyll fluorescence measurements show a partial photoinhibition of the photosynthetic apparatus, expressed by a decrease of the variable over maximal fluorescence ratio (Fv/Fm). This inhibition of Fv/Fm was significantly higher for plants grown at high-light intensity, leading to a photooxidation of chlorophyll. Newly formed leaves were better adapted to the ex vitro climatic condition (as shown by the increase of the Fv/Fm ratio) and had a higher net photosynthesis compared with in vitro formed leaves. Nevertheless, plants grown at 300 µmol·m−2·s−1 were photoinhibited, compared with those at 100 µmol·m−2·s−1. A sudden exposure to high-light intensity of 1-, 10- or 25-d-old transplanted plants (shift in PPFD from 100 to 300 µmol·m−2·s−1) gave a linear decrease of Fv/Fm over a 12-h period, which was reflected in a 50% reduction of net photosynthesis. No significant interaction between day and hour was found, indicating high-light exposure causes the same photoinhibitory effect on in vitro and ex vitro formed leaves.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arnon, D. I. Copper enzymes in isolated chloroplasts. Polyphenol oxidase inBeta vulgaris. Plant Physiol. 24:1–15; 1949.
Björkman, O. Low-temperature chlorophyll fluorescence in leaves and its relationship to photon yield of photosynthesis in photoinhibition. In: Kyle, D. J.; Osmond, C. B.; Arntzen, C. J., eds. Topics in photosynthesis: photoinhibition. Amsterdam, Netherlands: Elsevier Science Publishers; 1987:123–145.
Björkman, O.; Demmig, B. Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77K among vascular plants of diverse origins. Planta 170:489–504; 1987.
Capellades, M.; Fontarnau, R.; Carulla, C., et al. Environment influences anatomy of stomata and epidermal cells in tissue cultured Rosa multiflora. J. Am. Soc. Hortic. Sci. 115:141–145; 1990.
Chaves, M. M. Environmental constraints to photosynthesis in ex vitro plants. In: Lumsden, P. J.; Nicholas, J. R.; Davies, W. J., eds. Physiology, growth and development of plants in culture. Dordrecht, Netherlands: Kluwer Academic Publishers; 1994:1–18.
Desjardins, Y.; Gosselin, A.; Yelle, S. Acclimatization of ex vitro strawberry plantlets in CO2-enriched environments and supplementary lighting. J. Am. Soc. Hortic. Sci. 112:846–851; 1987.
Donnelly, D. J.; Vidaver, W. E. Leaf anatomy of red raspberry transferred from in vitro culture to soil. J. Am. Soc. Hortic. Sci. 109:172–176; 1984.
Donnelly, D. J.; Vidaver, W. E.; Lee, K. Y. The anatomy of tissue cultured red raspberry prior to and after transfer to soil. Plant Cell Tissue Organ Cult. 4:43–50; 1985.
Genty, B.; Briantais, J.-M.; Baker, N. R. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochem. Biophys. Acta 900:87–92; 1989.
Grout, B. W. W.; Aston, M. J. Transplanting of cauliflower plants regenerated from meristem culture. II. Carbon dioxide fixation and development of photosynthetic ability. Hortic. Res. 17:65–71; 1978.
Krause, G. H.; Weis, E. Chlorophyll fluorescence and photosynthesis: the basics. Annu. Rev. Plant Physiol. Plant Mol. Biol. 42:313–349; 1991.
Lee, N.; Wetzstein, H. Y.; Sommer, H. E. Effects of quantum flux density on photosynthesis and chloroplast ultrastructure in tissue-cultured plantlets and seedlings of Liquidambar styraciflua L. towards improved acclimatization and field survival. Plant Physiol. 78:637–641; 1985.
Ögren, E. Evaluation of chlorophyll fluorescence as a probe for drought stress in willow leaves. Plant Physiol. 93:1280–1285; 1990.
Öquist, G.; Anderson, J. M.; McCaffery, S., et al. Mechanistic differences in photoinhibition of sun and shade plants. Planta 188:422–431; 1992.
Osmond, C. B. What is photoinhibition? Some insights from comparisons of shade and sun plants. In: Baker, N. R.; Bowyer, J. R., eds. Photoinhibition of photosynthesis: from molecular mechanisms to the field. Oxfore, United Kingdom: BIOS Scientific Publishers Ltd.; 1994: 1–24.
Pospisilova, J.; Solarova, J.; and Catsky, J. Photosynthetic responses to stresses duringin vitro cultivation. Photosynthetica 26(1):3–18; 1992.
Preece, J. E.; Sutter, E. G. Acclimatization of micropropagated plants to the greenhouse and the fields. In: Debergh, P. C.; Zimmerman, R. H., eds. Micropropagation. Dordrecht, Netherlands: Kluwer Academic Publishers; 1991:71–93.
SAS Institute. SAS/STAT. User’s guide, release 6.03. SAS Institute Inc., Cary, NC; 1988: 1028p.
van Kooten, O.; Snel, J. F. H. The use of chlorophyll fluorescence nomenclature in plant stress physiology. Photosynth. Res. 25:147–150; 1990.
Yue, D.; Desjardins, Y.; Lamarre, M., et al. Photosynthesis and transpiration of in vitro cultured asparagus plantlets. Scientia Horticulturae 49:9–16; 1992.
Ziv, M. Vitrification: morphological and physiological disorders of in vitro plants. In: Debergh, P. C.; Zimmerman, R. H., eds. Micropropagation. Dordrecht, Netherlands: Kluwer Academic Publishers; 1991:45–69.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Van Huylenbroeck, J.M., Huygens, H. & Debergh, P.C. Photoinhibition during acclimatization of micropropagatedSpathiphyllum “Petite” plantlets. In Vitro Cell Dev Biol - Plant 31, 160–164 (1995). https://doi.org/10.1007/BF02632013
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02632013