Summary
Eucalyptus camaldulensis shoots were cultured photoautotrophicallyin vitro for 6 wk with four different types of supporting materials (agar matrix, Gelrite matrix, plastic net, or vermiculite) under CO2-nonenriched or CO2-enriched conditions. Plantlets from each treatmentin vitro were then grownex vitro in a greenhouse for 4 wk. The growth and net photosynthetic rate of plantletsin vitro, as well as subsequent growth, survival percentage, transpiration rate, and net photosynthetic rate of plantletsex vitro were evaluated. CO2 enrichment significantly increased growth (total dry weight and number of primary roots) and net photosynthetic rate of plantletsin vitro, as well as the growth and survival percentage of plantletsex vitro regardless of the type of supporting materials. The growthin vitro was greatest in the vermiculite, followed by the plastic net, Gelrite matrix, and agar matrix (in descending order) under either the CO2-nonenriched or CO2-enriched conditions. The growth and survival percentage of plantletsex vitro were highest in the vermicultie under the CO2-enriched condition. The extensive root system producedin vitro was necessary for growth and survival of plantletsex vitro.
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Kirdmanee, C., Kitaya, Y. & Kozai, T. Effects of CO2 enrichment and supporting materialin vitro on photoautotrophic growth ofEucalyptus plantletsin vitro andex vitro . In Vitro Cell Dev Biol - Plant 31, 144–149 (1995). https://doi.org/10.1007/BF02632010
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DOI: https://doi.org/10.1007/BF02632010