Summary
In the genusMusa, germination is extremely variable and relatively difficult. Even more difficulties are faced when producing hybrids. The seed yield of hybrids in breeding programs is usually low and often, to ensure the viability and survival of seeds, it is necessary to attempt to germinate a large excess of these seeds. In this context,in vitro embryo culture might be an invaluable tool for obtaining desirable hybrid plants in a short time. Seeds ofMusa velutina were sown in seed trays in a peat-based mixture. Thein vivo seed germination reached 78% but only after 9 mo. Because of this delayed and intermittent germination, embryos were excised from seeds and inoculated onto half-strength Murashige and Skoog (1962) medium, with or without supplementation with various concentrations of gibberellic acid. Light and dark conditions were also used to test their effect on embryo germination. After 2 wk, 82% of embryos germinated in the dark on medium containing 0.1 µM gibberellic acid. Addition of gibberellic acid increased the shoot length and root number over the gibberellic acid-free treatment. Similarly, dark conditions gave a significant increase over light conditions for all the parameters except root number where light or dark conditions did not make any difference. Thus, the present study highlights the importance of various components of thein vitro culture ofMusa embryos and the advantage over direct use of greenhouse-sown seeds both in terms of the time taken to germinate and the final percentage.
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References
Afele, J. C.; De Langhe, E. Increasingin vitro germination ofMusa balbisiana seed. Plant Cell Tissue Organ Cult. 27:33–36; 1991.
Buchheim, J. A. T., Burkhart, L. F.; Meyer, M. M., Jr. Effect of exogenous gibberellic acid, abscisic acid and benzylaminopurine on epicotyl dormancy of cultured herbaceous peony embryos. Plant Cell Tissue Organ Cult. 36:35–43; 1994.
Bulard, C.; Monin, J. Action de l’acid gibberellique sur des embryons dormantes deEuonymus europaeus cultivésin vitro. Compt. Rend. Acad. Sci. Paris 250:2922–2924; 1960.
Bulard, C.; Monin, J. Etude du compoitement d’embryons deFraxinus excelsior L. prélevés dans des graines dormantes et culktivésin vitro. Phyton 250:115–125; 1963.
Cox, E. A.; Stotzky, G.; Goos, R. D.In vitro culture ofMusa balbisiana colla embryos. Nature 185:403–404; 1960.
Hisajima, S.; Jong, F. S.; Arai, Y., et al. Propagation and breeding of sago palm (Metroxylon sp.) plantin vitro. 1. Embryo culture and induction of multiple shoots from sago embryosin vitro. Jpn. J. Trop. Agric. 35:259–267; 1991.
McGahan, M. W. Studies on the seed of banana. I. Anatomy of the seed and embryo ofMusa balbisiana. Am. J. Bot. 48:230–238; 1961.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Shepherd, K.; Dantas, J. L.; Alves, E. J. Banana breeding in Brazil. In: Persley, G. J.; De Langhe, E., eds. Banana and plantain breeding strategies. Proceedings of an international workshop. Cairns, Australia. ACIAR Proceedings no. 21:78–83; 1987.
Simmonds, N. W. The germination of banana seeds. Trop. Agric. 29:35–49; 1952.
Simmonds, N. W. Experiment on the germination of banana seeds. Trop. Agric. 36:259–273; 1959.
Sinska, I. Interaction of ethephon with cytokinin and gibberellin during the removal of apple seed dormancy and germination of embryos. Plant Sci. (Limerick) 64:39–44; 1989.
Stotzky, G.; Cox, E. A.; Goos, R. D. Seed germination studies inMusa. I. Scarification and aseptic germination ofMusa balbisiana. Am. J. Bot. 49:515–520; 1962.
van Beckum, J. M. M.; Libbenga, K. R.; Wang, M. Abscisic acid and gibberellic acid-regulated responses of embryos and aleurone layers isolated from dormant and non-dormant barley grains. Physiol. Plant. 89:483–489; 1993.
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Pancholi, N., Wetten, A. & Caligari, P.D.S. Germination ofMusa velutina seeds: Comparison ofin vivo andin vitro systems. In Vitro Cell Dev Biol - Plant 31, 127–130 (1995). https://doi.org/10.1007/BF02632006
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DOI: https://doi.org/10.1007/BF02632006