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Effects of differentiation of embryonal carcinoma cells (P19) on mitochondrial DNA content in vitro

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Summary

The embryonal carcinoma cell line P19 is derived from mouse teratocarcinomas. These pluripotent cells can be induced to differentiate into a variety of cell types by exposure to various drugs. We used retinoic acid to induce embryonal carcinoma cells to differentiate into neuronlike cells. In this study, we show that changes occur in mitochondria during differentiation of embryonal carcinoma cells to neuronlike cells. We found that various morphologic parameters such as mitochondrial fractional area and mitochondrial size decrease as embryonal carcinoma cells differentiate into neuronlike cells. Similar changes were also observed in mitochondrial DNA content. Stereologic analysis of cell preparations provided a measure of mitochondrial fractional area per cell and mtDNA content was assessed by radiolabeled mtDNA probe. This study establishes that mitochondria are regulated as cells differentiate.

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References

  1. Bogenhagen, D.; Clayton, D. A. The number of mitochondrial deoxyribonucleic acid genomes in mouse L and human HeLa cells. J. Biol. Chem. 249:7991–7995; 1974.

    PubMed  CAS  Google Scholar 

  2. Collins, J. M.; Foster, K. A. Differentiation of promyelocytic (HL-60) cells into mature granulocytes; mitochondrial-specific Rhodamine 123 fluorescence. J. Cell Biol. 96:94–99; 1983.

    Article  PubMed  CAS  Google Scholar 

  3. Goto, K.; Hayashi, S.; Shirayoshi, Y., et al. Exogenous δ-crystallin gene expression as probe for differentiation of teratocarcinoma stem cells. Cell Differ. 24:139–148; 1988.

    Article  PubMed  CAS  Google Scholar 

  4. Jones-Villeneuve, E. M. V.; McBurney, M. W.; Rogers, K. A., et al. Retinoic acid induces embryonal carcinoma cells to differentiate into neurons and glial cells. J. Cell Biol. 94:253–262; 1982.

    Article  PubMed  CAS  Google Scholar 

  5. Jones-Villeneuve, E. M. V.; Rudnicki, M. A.; Harris, J. F., et al. Retinoic acid-induced neural differentiation of embryonal carcinoma cells. Mol. Cell. Biol. 3:2271–2279; 1983.

    PubMed  CAS  Google Scholar 

  6. McBurney, M. W.; Jones-Villeneuve, E. M. V.; Edwards, M. K. S., et al. Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line. Nature 299:165–167; 1982.

    Article  PubMed  CAS  Google Scholar 

  7. McBurney, M. W.; Rogers, B. J. Isolation of male embryonal carcinoma cells and their chromosome replication patterns. Dev. Biol. 89:503–508; 1982.

    Article  PubMed  CAS  Google Scholar 

  8. McBurney, M. W.; Reuhl, K. R.; Ally, A. I., et al. Differentiation and maturation of embryonal carcinoma-derived neurons in cell culture. J. Neurosci. 8:1063–1073; 1988.

    PubMed  CAS  Google Scholar 

  9. Nass, M. M. K. Mitochondrial DNA: advances, problems and goals. Science 165:25–35; 1969.

    Article  CAS  Google Scholar 

  10. Papaioannou, V. E. Interactions between mouse embryos and teratocarcinomas. INSERM (Int. Nat. Sante Rech. Med.) Symp. 10:141–155; 1979.

    Google Scholar 

  11. Rudnicki, M. A.; McBurney, M. W. Cell culture methods and induction of differentiation of embryonal carcinoma cell lines. In: Robertson, E. J., ed. Teratocarcinomas and embryonic stem cells: a practical approach. Oxford, England: IRL Press; 1987:19–49.

    Google Scholar 

  12. Sporn, M. B.; Roberts, A. B.; Goodman, D. S. The retinoids, vols. 1,2. New York: Academic Press; 1984.

    Google Scholar 

  13. Spurr, A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26:31; 1969.

    Article  PubMed  CAS  Google Scholar 

  14. Strickland, S.; Mahdavi, V. The induction of differentiation in teratocarcinoma stem cells by retinoic acid. Cell 15:393–403; 1978.

    Article  PubMed  CAS  Google Scholar 

  15. Veltri, K. L.; Espiritu, M.; Singh, G. Distinct genomic copy number in mitochondria of different mammalian organs. J. Cell. Physiol. 143:160–164; 1990.

    Article  PubMed  CAS  Google Scholar 

  16. Williams, R. S.; Salmons, S.; Newholme, E. A., et al. Regulation of nuclear and mitochondrial gene expression by contractile activity in skeletal muscle. J. Biol. Chem. 261:376–380; 1986.

    PubMed  CAS  Google Scholar 

  17. Williams, R. S. Mitochondrial gene expression in mammalian striated muscle. J. Biol. Chem. 261:12390–12394; 1986.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, Ontario Cancer Foundation, Hamilton Regional Cancer Centre and McMaster University, 1200 Main Street West, L8N 3Z5, Hamilton, Ontario, Canada

    Gurmit Singh & Karen L. Veltri

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  1. Gurmit Singh
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  2. Karen L. Veltri
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This study was financially supported by the Medical Research Council of Canada.

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Singh, G., Veltri, K.L. Effects of differentiation of embryonal carcinoma cells (P19) on mitochondrial DNA content in vitro. In Vitro Cell Dev Biol - Animal 27, 557–561 (1991). https://doi.org/10.1007/BF02631286

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  • DOI: https://doi.org/10.1007/BF02631286

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