References
Branch, D. R.; Guilbert, L. J. Practical in vitro assay systems for the measurement of hematopoietic growth factors. J. Tissue Culture Methods 10:101–108; 1986.
Chen, T. R.In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp. Cell Res. 104:255–262; 1977.
Dabbs, D. J. W.; Limb, D. I.; Spencer, R. C. The new fluoroquinolones: a review. Med. Lab. Sci. 44:59–65; 1987.
Gignac, S. M.; Brauer, S.; Häne, B., et al. Elimination of mycoplasma from infected leukemia cell lines. Leukemia 5:162–165; 1991.
Hessling, J. J.; Miller, S. E.; Levy, N. L. A direct comparison of procedures for the detection of mycoplasma in tissue culture. J. Immunol. Methods 38:315–324; 1980.
Jeansson, S.; Brorson, J. E. Elimination of mycoplasmas from cell cultures utilizing hyperimmune sera. Exp. Cell Res. 161:181–188; 1985.
Kotani, H.; Butler, G.; Heggan, D., et al. Elimination of mycoplasmas from cell cultures by a novel soft agar technique. In Vitro Cell. Dev. Biol. 27:509–513; 1991.
Marcus, M.; Lavi, U.; Nattenberg, A., et al. Selective killing of mycoplasmas from contaminated mammalian cells in cell cultures. Nature 285:659–661; 1980.
Mowles, J. M. The use of ciprofloxacin for the elimination of mycoplasma from naturally infected cell lines. Cytotech. 1:355–358; 1988.
Nair, C. N. Elimination of mycoplasma contaminants from cell cultures with animal serum. Proc. Soc. Exp. Biol. Med. 179:254–258; 1985.
Ravaoarinoro, M.; Lecomte, J. Evaluation of three methods for curing hybridomas from mycoplasma contamination. Hybridoma 7:79–86; 1988.
Schmidt, J.; Erfle, V. Elimination of mycoplasmas from cell cultures and establishment of mycoplasma-free cell lines. Exp. Cell Res. 152:565–570; 1984.
Schmitt, K.; Däubener, W.; Bitter-Suermann, D., et al. A safe and efficient method for elimination of cell culture mycoplasmas using ciprofloxacin. J. Immunol. Methods 109:17–25; 1988.
Stanbridge, E. J. Mycoplasma detection—an obligation to scientific accuracy. Isr. J. Med. Sci. 17:563–568; 1981.
Shoenfeld, Y.; Schwartz, R. S. The production of monoclonal antibodies by human-human hybridomas: their application to studies of autoimmune diseases. In: Engleman, E. G.; Foung, S. K. H.; Larrick, J., et al., eds. Human hybridomas and monoclonal antibodies. New York: Plenum Press; 1985:247–262.
van Diggelen, O. P.; Shin, S.; Phillips, D. M. Reduction in cellular tumorigenicity after mycoplasma infection and elimination of mycoplasma from infected cultures by passage in nude mice. Cancer Res. 37:2680–2687; 1977.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Somasundaram, C., Nicklas, W. & Matzku, S. Use of ciprofloxacin and bm-cyclin in mycoplasma decontamination. In Vitro Cell Dev Biol - Animal 28, 708–710 (1992). https://doi.org/10.1007/BF02631057
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02631057