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Secretion of polypeptides related to epidermal growth factor and insulinlike growth factor I by a human teratocarcinoma cell line

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Summary

To identify polypeptide growth factors for human teratocarcinoma cells, we studied the malignant ovarian teratoma-derived cell line, PA-1, that grew autonomously in serum-free medium. Medium conditioned by undifferentiated PA-1 cells strongly stimulated proliferation of the mouse mammary tumor cell line, GR 2H6, which is responsive to epidermal growth factor (EGF) and insulinlike growth factor-I (IGF-I). After ammonium sulfate precipitation, PA-1 conditioned medium was analyzed by anion exchange chromatography and bioassay of elution fractions on GR 2H6 cells that were grown in medium deficient in either EGF or insulin. The results demonstrated that PA-1 CM contained factors that can substitute for EGF and IGF-I in stimulating growth of GR 2H6 cells. Western blots of peak mitogenic fractions revealed low molecular weight polypeptides that were immunoreactive with either anti-EGF or anti-IGF-I antibodies. Indirect immunofluorescence staining of PA-1 cells with monoclonal antibodies localized receptors for each growth factor, and binding of human EGF and IGF-I to these cells was quantified by radioreceptor assays. Secretion of factors closely related to EGF and IGF-I by PA-1 cells under serum-free conditions may provide a novel model system to study molecular mechanisms of autocrine growth stimulation in teratocarcinomas.

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Author notes

  1. Hiroshi Kitani

    Present address: National Institute of Animal Health, 3-1-1 Kannondai, 305, Tsukuba, Ibaraki, Japan

  2. Hisaaki Kawakatsu

    Present address: Nippon Shinyaku Co., Ltd., Nishiohji Hachijo, Minamiku, 601, Kyoto, Japan

Authors and Affiliations

  1. Laboratory of Cell Biology, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, 305, Tsukuba, Ibaraki, Japan

    Naihe Jing, Robert Shiurba, Hiroshi Kitani, Hisaaki Kawakatsu, Yasuhiro Tomooka & Teruyo Sakakura

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  1. Naihe Jing
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Jing, N., Shiurba, R., Kitani, H. et al. Secretion of polypeptides related to epidermal growth factor and insulinlike growth factor I by a human teratocarcinoma cell line. In Vitro Cell Dev Biol - Animal 27, 864–872 (1991). https://doi.org/10.1007/BF02630989

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