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Polarized secretion of tissue-plasminogen activator in cultured thyroid cells

  • Growth, Differentiation And Senescence
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Summary

We studied the polarized secretion of tissue-type plasminogen activator in porcine thyroid cells cultured as a monolayer on porous bottom chambers. The presence of tissue-type plasminogen activator was detected by zymographic analysis on two independent media that were in contact either with the apical surface or with the basolateral membrane. The amount of tissue-type plasminogen activator was determined in both media by ELISA and enzyme assay. Measurable tissue-type plasminogen activator activity was found in the basal but not in the apical medium. However, on zymogram, a lytic zone corresponding to tissue-type plasminogen activator was visible in both media. In addition, a lytic band at 130 kDa suggested presence of a complex formed by tissue-type plasminogen activator and an inhibitor. Preferential basolateral tissue-type plasminogen activator antigen secretion (70%) has been observed, showing the possible relation between tissue-type plasminogen activator and extracellular matrix components. Neither tissue-type plasminogen activator level nor polarized secretion seemed to be regulated by thyrotropin (0.1 mU/ml).

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Author notes

  1. P. Delori

    Present address: Délégation Régionale CNRS, Sophia-Antipolis, 06560, Valbonne, France

Authors and Affiliations

  1. Inserm U.270, Faculté de médecine Nord, Bd. P. Dramard, 13916, Marseille cedex 20, France

    S. Desruisseau-Gonzalvez, P. Delori, D. Gruffat & O. Chabaud

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  1. S. Desruisseau-Gonzalvez
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  2. P. Delori
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  3. D. Gruffat
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  4. O. Chabaud
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Desruisseau-Gonzalvez, S., Delori, P., Gruffat, D. et al. Polarized secretion of tissue-plasminogen activator in cultured thyroid cells. In Vitro Cell Dev Biol - Animal 29, 161–164 (1993). https://doi.org/10.1007/BF02630948

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  • DOI: https://doi.org/10.1007/BF02630948

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