Summary
Coculture of two different cell lines in monolayer or spheroids was used to investigate the spontaneous transfer of dominant genes determining drug resistance. MGH-U1 human bladder cancer cells (ouabain-sensitive, mitomycin C-resistant) were cocultured with UV-20 cells (a subline of Chinese hamster ovary cells which is ouabain-resistant and mitomycin C-sensitive). We investigated the possible transfer of mitomycin-C resistance from human to rodent cells by selection in both ouabain and mitomycin C. Regardless of coculture conditions, the frequency of surviving cells was at a similar level to that expected from studies of cell survival when cells were cultured alone. We found no evidence of spontaneous transfer of drug resistance between the two cell lines.
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Luk, C.K., Tannock, I.F. Evaluation of the probability of spontaneous transfer of drug resistance between cells in culture. In Vitro Cell Dev Biol - Animal 27, 245–248 (1991). https://doi.org/10.1007/BF02630924
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DOI: https://doi.org/10.1007/BF02630924