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Culture requirements for optimal expression of 1α,25-dihydroxyvitamin D3-enhanced thyrotropin secretion

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Summary

An effect of the hormone, 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3] on hormone secretion by normal rat pituitary cells was investigated in vitro. Based on previous findings using GH4C1 cells, dispersed anterior pituitary cell cultures were prepared and maintained in serum-free conditions for up to 6 d. Under these circumstances, there was no effect of 1,25(OH)2D3 to alter medium or cell-associated levels of thyrotropin (TSH), prolactin (PRL), or growth hormone (GH). Cultures maintained under these conditions had lower medium and cell-associated hormone levels and lesser responses to agonists than cultures maintained in serum-supplemented medium. In the presence of 10% charcoal-treated fetal bovine serum, treatment with 10−8 M 1,25(OH)2D3 for 24 h selectively increased TRH (10−10 to 10−7 M)-induced TSH secretion (P<0.001), with maximal enhancement observed at 10−9 M TSH-releasing hormone (TRH). Enhancement of TSH secretion by 1,25(OH)2D3 was detected after 15 min exposure to TRH. There was no effect on agonist-induced PRL or GH secretion or on cell-associated hormone levels. The effect was evident after 24 h treatment with 1,25(OH)2D3, and decreased thereafter. Several other steroid hormones had no effect on 10−9 M TRH-induced TSH secretion. These data contrast with the effect of 1,25(OH)2D3 in GH cells. They suggest that 1,25(OH)2D3 may act selectively in the normal pituitary to modulate TSH secretion.

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References

  1. Baker, R. J.; Nelder, J. A. Generalised linear model. In GLIM manual, release 3. Oxford: Numerical Algorithms Group; 1978.

    Google Scholar 

  2. Bauer, R. F.; Arthur, L. O.; Fine, D. L. Propagation of mouse mammary tumor cell lines and production of mouse mammary tumor virus in a serum-free medium. In Vitro 12:558–563; 1976.

    PubMed  CAS  Google Scholar 

  3. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248–254; 1976.

    Article  PubMed  CAS  Google Scholar 

  4. d’Emden, M. C.; Wark, J. D. 1,25-Dihydroxyvitamin D3 enhances thyrotropin-releasing hormone induced thyrotropin secretion in normal pituitary cells. Endocrinology 121:1192–1194; 1987.

    PubMed  CAS  Google Scholar 

  5. Drouin, J.; Lagace, L.; Labrie, F. Estradiol-induced increase of the LH responsiveness to LH releasing hormone in rat anterior pituitary cells in culture. Endocrinology 99:1477–1481; 1976.

    Article  PubMed  CAS  Google Scholar 

  6. Gershengorn, M. C. Thyrotropin releasing hormone action: mechanisms of calcium-mediated stimulation of prolactin secretion. Rec. Prog. Horm. Res. 41:607–653; 1985.

    PubMed  CAS  Google Scholar 

  7. Ham, R. G. Nutritional requirements of primary cultures. A neglected problem of modern biology. In Vitro 10:119–129; 1974.

    Article  PubMed  CAS  Google Scholar 

  8. Haug, E.; Pedersen, J. I.; Gautvik, K. M. Effects of vitamin D3 metabolites on production of prolactin and growth hormone in rat pituitary cells. Mol. Cell. Endocrinol. 28:65–71; 1982.

    Article  PubMed  CAS  Google Scholar 

  9. Haug, E.; Bjoro, T.; Gautvik, K. M. A permissive role for extracellular Ca2+ in regulation of prolactin production by 1,25-dihydroxyvitamin D3 in GH3 pituitary cells. J. Steroid Biochem. 28:385–391; 1987.

    Article  PubMed  CAS  Google Scholar 

  10. Haussler, M. R.; Manolagas, S. C.; Deftos, L. J. Receptor for 1,25-dihydroxyvitamin D3 in GH3 pituitary cells. J. Steroid Biochem. 16:15–19; 1982.

    Article  PubMed  CAS  Google Scholar 

  11. Maurer, R. A. Dopaminergic inhibition of prolactin synthesis and prolactin messenger RNA accumulation in cultured pituitary cells. J. Biol. Chem. 255:8092–8097; 1980.

    PubMed  CAS  Google Scholar 

  12. Maurer, R. A. Relationship between estradiol, ergocriptine, and thyroid hormone: effects on prolactin synthesis and prolactin messenger ribonucleic acid levels. Endocrinology 110:1515–1520; 1982.

    PubMed  CAS  Google Scholar 

  13. Miller, R.; Horn, A.; Iversen, L. Effects of dopamine-like drugs on rat striatal adenyl cyclase have implications for CNS dopamine receptor topography. Nature 250:238–241; 1974.

    Article  PubMed  CAS  Google Scholar 

  14. Murdoch, G. H.; Rosenfeld, M. G. Regulation of pituitary function and prolactin production in the GH4 cell line by vitamin D. J. Biol. Chem. 256:4050–4055; 1981.

    PubMed  CAS  Google Scholar 

  15. Norman, A. W.; Roth, J.; Orci, L. The vitamin D endocrine system: steroid metabolism, hormone receptors, and biological response (calcium binding proteins). Endocr. Rev. 3:331–366; 1982.

    Article  PubMed  CAS  Google Scholar 

  16. Raymond, V.; Beaulieu, M.; Labrie, F. Potent antidopaminergic activity of estradiol at the pituitary level on prolactin release. Science 200:1173–1175; 1978.

    Article  PubMed  CAS  Google Scholar 

  17. Reinhardt, T. A.; Horst, R. I.; Orf, J. W., et al. A microassay for 1,25-dihydroxyvitamin D not requiring high performance liquid chromatography:applications to clinical studies. J. Clin. Endocrinol. Metab. 58:91; 1984.

    PubMed  CAS  Google Scholar 

  18. Sar, M.; Stumpf, W. E.; DeLuca, H. F. Thyrotrophs in the pituitary are target cells for 1,25 dihydroxyvitamin D3. Cell Tissue Res. 209:161–166; 1980.

    Article  PubMed  CAS  Google Scholar 

  19. Sar, M.; Miller, W. L.; Stumpf, W. E. Effects of 1,25 dihydroxyvitamin D3 on thyrotropin secretion in vitamin D deficient male rats. Physiologist 24:70; 1981.

    Google Scholar 

  20. Shiota, K.; Yoshida, K.; Matasi, T., et al. Biphasic release of thyrotropin in response to thyrotropin releasing hormone (TRH) from rat anterior pituitary cellsin vitro: possible dependence on protein synthesis. Endocrinol Jpn. 31:165–175; 1984.

    PubMed  CAS  Google Scholar 

  21. Shull, J. D.; Gorski, J. The hormonal regulation of prolactin gene expression: an explanation of mechanisms controlling prolactin synthesis and possible relationship of estrogen to these mechanism. Physiol. Rev. 36:197–249; 1986.

    Google Scholar 

  22. Stumpf, W. E.; Sar, M.; O’Brien, L. P. Vitamin D sites of action in the pituitary studied by combined autoradiography-immunohistochemistry. Histochemistry 88:11–16; 1987.

    Article  PubMed  CAS  Google Scholar 

  23. Tornquist, K.; Lamberg-Allardt, C. Effect of 1,25(OH)2D3, verapamil and EDTA-infusion on the thyroliberin-induced thyrotropin release. In: Norman, A. W., eds. Vitamin D. A chemical, biochemical and clinical update. Berlin: Walter de Gruyter & Co.; 1985:363–364.

    Google Scholar 

  24. Vale, W.; Vaughan, J.; Yamamoto, G., et al. Effects of synthetic human pancreatic (tumor) GH releasing factor and somatostatin, triiodothyronine and dexamethasone on GH secretionin vitro. Endocrinology 112:1553–1555; 1983.

    PubMed  CAS  Google Scholar 

  25. Verbeelen, D.; Vanhaelst, L.; Fuss, M., et al. Effect of 1,25-dihydroxyvitamin D3 and nifedipine on prolactin release in normal man. J. Endocrinol. Invest. 8:103–106; 1985.

    PubMed  CAS  Google Scholar 

  26. Wark, J. D.; Tashjian, A. H., Jr. Vitamin D stimulates prolactin synthesis by GH4C1 cells incubated in chemically defined medium. Endocrinology 111:1755–1757; 1982.

    PubMed  CAS  Google Scholar 

  27. Wark, J. D.; Tashjian, A. H., Jr. Regulation of prolactin mRNA by 1,25-dihydroxyvitamin D3 in GH4C1 cells. J. Biol. Chem. 258:12118–12121; 1983.

    PubMed  CAS  Google Scholar 

  28. Wark, J. D.; Gurtler, V. Glucocorticoids antagonise induction of prolactin gene expression by calcitriol in rat pituitary tumour cells. Biochem. J. 233:513–518; 1986.

    PubMed  CAS  Google Scholar 

  29. Woodruff, G. N.; Watling, K. J.; Andrews, C. D., et al. Dopamine receptors in rat striatum and nucleus accumbens: conformational studies using rigid analogues of dopamine. J. Pharm. Pharmac. 29:422–427; 1977.

    CAS  Google Scholar 

  30. Zofkova, I.; Bednar, J. Effect of 1,25(OH)2 vitamin D3 (calcitriol) on TRH-induced thyrotropin secretion in man. Exp. Clin. Endocrinol. 91:7–12; 1988.

    Article  PubMed  CAS  Google Scholar 

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  1. Department of Medicine, University of Melbourne, Royal Melbourne Hospital, 3050, Parkville, Victoria, Australia

    Michael C. d’Emden & John D. Wark

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  1. Michael C. d’Emden
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  2. John D. Wark
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d’Emden, M.C., Wark, J.D. Culture requirements for optimal expression of 1α,25-dihydroxyvitamin D3-enhanced thyrotropin secretion. In Vitro Cell Dev Biol - Animal 27, 197–204 (1991). https://doi.org/10.1007/BF02630916

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