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Increase in CD57+CD16-lymphocytes in workers exposed to benzidine and beta-naphthylamine: assessment of natural killer cell subpopulations

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Abstract

Previously, we found a decrease in CD4+CD45RA+T lymphocytes in workers exposed to the aromatic amines (AAs) [benzidine (BZ) and beta naphthylamine (BNA)]. For further investigation of the effects of AAs on lymphocyte subpopulations, we measured natural killer (NK) cell subpopulations using two-color staining with anti-Leu7 (CD57) and anti-Leu11 (CD16) monoclonal antibodies in peripheral blood in 78 male dyestuff workers. The workers had been exposed to AAs before 1972 at a chemical plant, either in the production of AAs (40 workers, high-exposure group) or in other work that involved handling dye-stuffs (38 workers, low-exposure group). The controls were 30 “healthy” male volunteers without a history of occupational exposure to AAs or hazardous chemicals. The number of CD57+CD16-cells in the high-exposure group was significantly higher than that in the controls (P<0.01, analysis of covariance with age as a covariate). No significant differences were found in CD57+CD16-, CD57+CD16+ and CD57-CD16+NK cells between the low-exposure group and the controls. It is suggested that a decrease in the number of CD4+ T lymphocytes following exposure to AAs might be compensated by the increase in CD57+CD16- cells, i.e. circulating peripheral lymphocytes with poor NK cell activity.

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References

  1. Abo T (1992) Extrathymic differentiation of T lymphocytes and its biolgoical function. Biomed Res 13:1–39

    CAS  Google Scholar 

  2. Abo T, Balch CM (1981) A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J Immunol 127:1024–1029

    PubMed  CAS  Google Scholar 

  3. Abo T, Cooper MD, Balch CM (1982) Characterization of HNK-1+(Leu-7) human lymphocytes. I. Two distinct phenotypes of human NK cells with different cytotoxic capability. J Immunol 129:1752–1757

    PubMed  CAS  Google Scholar 

  4. Abo T, Miller CA, Balch CM (1984) Characterization of human granular lymphocyte subpopulations expressing HNK-1 (Leu-7) and Leu-11 antigens in the blood and lymphoid tissues from fetuses, neonates and adults. Eur J Immunol 14:616–623

    Article  PubMed  CAS  Google Scholar 

  5. Araki S, Tanigawa T, Ishizu S, Minato N (1993) Decrease of CD4 positive T lymphocytes in workers exposed to benzidine and beta-napthylamine. Arch Environ Health 48:205–208

    Article  PubMed  CAS  Google Scholar 

  6. Bakke AC, Gray JD, Abo W, et al (1986) Studies on human blood lymphocyte with iC3b (Type 3) complement receptors. I. Granular, Fc-IgG receptor positive and negative subsets in healthy subjects and patients with systemic lupus erythematosus. J Immunol 136:1253–1259

    PubMed  CAS  Google Scholar 

  7. Case RAM, Hosker ME, McDonald DB, Pearson JT (1954) Tumours of the urinary bladder in workmen engaged in the manufacture and use of certain dyestuff intermediates in the British chemical industry; the role of aniline, benzidine alpha-naphthylamine, and beta-naphthylamine. Br J Ind Med 11:75–104

    PubMed  CAS  Google Scholar 

  8. Ferrante A, Kiroff GK, Drew PA (1986) Elevated natural killer (NK) cytotoxicity of mononuclear leukocytes from splenectomized patients: increase in Leu−7+ and Leu−11+ leukocytes. Clin Exp Immunol 64:173–180

    PubMed  CAS  Google Scholar 

  9. Herberman RB, Ortaldo JR (1981) Natural killer cells: their role in defenses against disease. Science 214:24–30

    Article  PubMed  CAS  Google Scholar 

  10. Huwyler T, Morell A, Hirt A, et al, (1984) Correlation of natural killer cell function with Leu7 reactivity in patients with humoral immunodeficiency. Clin Exp Immunol 56:486–492

    PubMed  CAS  Google Scholar 

  11. International Agency for Research on Cancer (1987) Overall evaluations of carcinogenicity: an updating of IARC monographs volume 1 to 42. IARC Monogr Suppl 4:123–5 261–3

    Google Scholar 

  12. Kelemen E, Gergely P, Lebhoczky D, et al (1986) Permanent large granular lymphocytosis in the blood of splenectomized individuals without concomitant increase of in vitro natural killer cell cytotoxicity. Clin Exp Immunol 63:696–702

    PubMed  CAS  Google Scholar 

  13. Krishnaraj R, Blandford G (1988) Age-associated alterations in human natural killer cells: increased frequency of selective NK subsets. Cell Immunol 114:137–148

    Article  PubMed  CAS  Google Scholar 

  14. Lanier LL, Phillips JH (1985) A map of the cell surface antigens expressed on resting and activated human natural killer cells. In: Reinherz EL et al (eds) Leukocyte typing II. Springer, New York Berlin Heidelberg, pp 157–170

    Google Scholar 

  15. Lanier LL, Le AM, Phillips JH, Warner NL, Babcock GF (1983) Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol 131:1789–1796

    PubMed  CAS  Google Scholar 

  16. Legac E, Autran B, Merle-Beral H, Katlama C, Debre P (1992) Cd4+ CD7- CD57+ T cells: a new T-lymphocyte subset expanded during human immunodeficiency virus infection. Blood 79:1746–1753

    PubMed  CAS  Google Scholar 

  17. Legendre CM, Guttmann RD, Shu HH, Jean R (1985) Two-color immunofluorescence and flow cytometry analysis of lymphocytes in long-term renal allotransplant recipients: identification of a major Leu-7+/Leu3+ subpopulation. J Immunol 135:1061–1066

    PubMed  CAS  Google Scholar 

  18. Legendre CM, Yip GH, Rodrigues GA, Forbes RDC, Guttmann RD (1987) Characterization of an expanded large granular lymphocyte subset lacking natural killer activity present in renal allograft recipients. Transplantation 43:229–234

    Article  PubMed  CAS  Google Scholar 

  19. Leroy E, Calvo CF, Divine M, et al (1986) Persistence of T8+/HNK-1+ suppressor lymphocytes in the blood of long-term surviving patients after allogeneic bone marrow transplantation. J Immunol 137:2180–2189

    PubMed  CAS  Google Scholar 

  20. Ligthart GJ, Vlokhoven PC, Schuit HRE, Hijmans W (1986) The expanded null cell compartment in aging: increase in the number of natural killer cells and changes in T-cell and NK-cell subsets in human blood. Immunology 59:353–357

    PubMed  CAS  Google Scholar 

  21. Phillips JJ, Babcock GF (1983) NKP-15: a monoclonal antibody reactive against purified human natural killer cells and granulocytes. Immunol Lett 6:143–149

    Article  PubMed  CAS  Google Scholar 

  22. Sawada H, Abo T, Sugawara S, Kumagai K (1988) Prerequisite for the induction of lymphokine-activated killer cells from T lymphocytes. J Immunol 140:3668–3673

    PubMed  CAS  Google Scholar 

  23. Seki S, Abo T, Masuda T, Ohteki T, et al (1990) Identification of activated T cell receptor gamma delta lymphocytes in the liver of tumor-bearing hosts. J Clin Invest 86:409–415

    PubMed  CAS  Google Scholar 

  24. Seki S, Abo T, Sugiura K, et al (1991) Reciprocal T cell responses in the liver and thymus of mice injected with syngeneic tumor cells. Cell Immunol 137:46–60

    Article  PubMed  CAS  Google Scholar 

  25. Sung HL, Araki S, Tanigawa T, Sakurai S (1995) Selective decrease of the suppressor inducer (CD4+CD45RA+) T lymphocytes in workers exposed to benzidine and beta-naphthylamine. Arch Environ Health 50:196–199

    Article  CAS  Google Scholar 

  26. Tanigawa T, Araki S, Ishizu S, Motita T, Okazaki H, Minato N (1990) Natural killer cell activity in workers exposed to benzidine and beta-naphthylamine. Br J Ind Med 47:338–341

    PubMed  CAS  Google Scholar 

  27. Trinchieri G (1990) Biology of natural killer cells. Adv Immunol 47:187–376

    Article  Google Scholar 

  28. Tsuchiya K, Okubo T, Ishizu S (1975) An epidemiological study of occupational bladder tumours in the dye industry of Japan. Br J Ind Med 32:203–209

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Public Health, Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, 113, Tokyo, Japan

    Takeshi Tanigawa & Shunichi Araki

  2. Department of Medical Zoology and Immunology, Niigata University School of Medicine, 951, Niigata, Japan

    Toru Abo

  3. Clinical Laboratory, University of Tokyo Hospital, Hongo, Bunkyo-ku, 113, Tokyo, Japan

    Susumu Sakurai

Authors
  1. Takeshi Tanigawa
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  2. Shunichi Araki
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  3. Toru Abo
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  4. Susumu Sakurai
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Tanigawa, T., Araki, S., Abo, T. et al. Increase in CD57+CD16-lymphocytes in workers exposed to benzidine and beta-naphthylamine: assessment of natural killer cell subpopulations. Int. Arch Occup Environ Heath 69, 69–72 (1996). https://doi.org/10.1007/BF02630742

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  • DOI: https://doi.org/10.1007/BF02630742

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