Summary
Two lysosomal glycohydrolases, β-galactosidase and β-N-hexosaminidase which have been associated with kidney disease were measured in the urine of 110 youngsters with juvenile diabetes mellitus. The mean enzyme excretions in the diabetic group were intermediate between those of normal youngsters and those with active renal disease. Three youngsters with known kidney disease had elevations comparable to others in the diabetic group but no direct correlation could be shown between enzyme elevations and proteinuria or Addis count abnormalities. Positive correlations were seen between urine enzyme levels and indices of metabolic balance including blood sugar, cholesterol and triglycerides but not with urine sugar or ketones. Duration and estimated stage and control of diabetes also correlated with the urinary enzymes. These preliminary studies are consistent with the possibility that the excretion of these enzymes reflects the ongoing renal damage which occurs in most juvenile diabetics.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Belfiore F., Becchio L. L., Napoli E., Borzi V.: Increased β-N-acetyl-glucosaminidase activity in diabetes mellitus — Clin. Chem.20, 1229, 1974.
Bomback F. M., Nakagawa S., Kumin S., Nitowsky H.: Altered lysosomal glycohydrolase activities in juvenile diabetes mellitus — Diabetes25, 420, 1976.
Bosnes R. W., Taussky H. H.: On the colorimetric determination of creatinine by the Jaffe reaction — J. biol. Chem.158, 581, 1954.
Cahill G. R., Etzwiler D. D., Freinkel N.: Blood glucose control in diabetes — Diabetes25, 237, 1976.
Dance N., Price R. G., Cattell W. R., Lansdell T., Richards B.: The excretion of N-acetyl-β-glucosaminidase and β-galactosidase by patients with renal disease — Clin. chim. Acta27, 87, 1970.
Dance N., Price R. G., Robinson D., Sterling J. L.: β-galactosidase, β-glucosidase and N-acetyl-β-glucosaminidase in human kidney — Clin. chim. Acta24, 189, 1969.
Erickson R. P., Sandman R., Epstein C. J., Robertson W., Van B.: Lack of relationship between blood and urine levels of glycosaminoglycans and lysosomal enzymes — Biochem. Med.12, 331, 1975.
Francois R.: Reflexions sur le traitement du diabete insulino-dependant — Arch. franç. Pédiat.33, 425, 1976.
Fushimi H., Tarui S.: β-glycosidases and diabetic microangiopathy: I. Decreases of β-glycosidase activities in diabetic rat kidneys. II. An insulin dependent isozyme of β-N-acetyl-glucosaminidase — J. Biochem. (Tokyo)79, 265, 1976.
Gielow L., Maruhn D., Fuchs I., Mues G., Bock K. D.: Urinary excretion of lysosomal enzymes in essential hypertension. In:Peeters H. (Ed.): Protides of biological fluids. Pergamon Press, Oxford, Vol.21, 1974; p. 547.
Jackson R. L., Guthrie R. A., Esterly J., Biliginturan N., Yeast J., Saathoff J., Guthrie D. W.: Muscle capillary basement membrane changes in normal and diabetic children — Diabetes24 (Suppl. 2), 400, 1975.
Keiding N. R., Root H. F., Marble A.: Importance of control of diabetes in prevention of vascular complications — J. Amer. med. Ass.150, 964, 1952.
Kohler E.: Diabetic day. Setting goals for child-directed ambulatory program — Clin. Pediat. (Phila.)17, 24, 1978.
Kuwahara M. D., Lyons S. A., Rosenblit P. D., Metzger R. P.: Effect of streptozotocin diabetes on selected enzymatic activities in rat urine — Proc. Soc. exp. Biol. (N.Y.)153, 305, 1976.
Maruhn D., Rosenstiel K., Kennewis K., Parr D.: Urinary excretion of β-glucuronidase, N-acetyl-β-glycosaminidase, α-glucosidase and trehalose in diabetes mellitus — Klin. Chem. Biochemie5, 263, 1974.
Miller B. F., Keyes F. P., Curreir P. W.: Increase of serum β-glucuronidase activity in human diabetes mellitus — J. Amer. med. Ass.195, 189, 1966.
Österby R.: Electron microscopy of vascular changes in juvenile diabetes. In:Laron Z. (Ed.) Diabetes in juveniles. Modern problems in pediatrics. Vol. 12, S. Karger, Basel, 1975; p. 300.
Sandman R., Mangoles R. M., Koontz S. L.: Urinary lysosomal glycosidases after renal allotransplantation: Correlation of enzyme excretion with allograft rejection and ischemia — Clin. chim. Acta45, 349, 1973.
Sheth K. J., Good T. A.: Urinary acidic hydrolases in renal disease in children — Clin. Nephrol.10, 151, 1978.
Spiro R. G.: Glycoproteins and diabetic microangiopathy. In:Marble A., White P., Bradley R. F., Krall P. (Eds.): Joslin’s diabetes mellitus. Lea & Febiger, Philadelphia, 1971; p. 146.
Sternberg M., Drupt F., Safar D. M., Moisy M., Leclerc M., Lagrue G., Milliez P.: Excretion of β-galactosidase, acid and alkaline phosphotase, amylase, aspartateamino transferase, lactic dehydrogenase activities in nocturnal urines in 35 hypertensive patients with stenosis, atrophy or thrombosis of renal artery — Kidney Int.3, 411 (Abstr.), June, 1973.
Tarui S., Saito Y., Fushimi H.: The progression of diabetic microangiopathy and lysosomal glycosidases. Clinical studies on Japanese diabetics and experimental approaches with the streptozotocin diabetic rats. In:Baba S., Goto Y., Fukui I. (Eds): Diaberes mellitus in Asia. Excerpta Medica, Amsterdam, 1976; p. 212.
Wellwood J. M., Ellis B. G., Price R. G., Hammond K., Thompson A. E., Jones N. F.: Urinary N-acetyl-β-d-glucosaminidase activities in patients with renal disease — Brit. med. J.3, 408, 1975.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kohler, E., Sheth, K.J. & Good, T.A. Urinary acidic glycohydrolases as an index of kidney damage in juvenile diabetes mellitus. Acta diabet. lat 16, 247–255 (1979). https://doi.org/10.1007/BF02629119
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02629119