Cytoplasmic mediation of malignancy

Editor's Statement This is the first description of cytoplasmic mediation of tumorigenesis. There is a clear indication that cytoplasmic elements play a role in the expression of the malignant phenotype.

Summary

The relative roles of nucleus and cytoplasm in the induction and maintenance of the malignant state were studied. Cytoplasmic hybrid (cybrid) clones, derived from the fusion of cytoplasts from malignantly transformed cells to normal whole cells, produced tumors in 17% of the animals injected with them. Nuclear/cytoplasmic hybrid (reconstituted cell) clones, derived by fusion of cytoplasts from malignant cells with karyoplasts of normal cells, produced tumors in 97% of the animals injected. A unique aspect of this study is the fact that all of the cells utilized, both normal and malignantly transformed, were derived from an original cloned cells

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Correspondence to Warren I. Schaeffer.

Additional information

This work was supported in part by National Cancer Institute Grant RO1-CA40206 from the National Institutes of Health. BAI was suported, in part, by a predoctoral fellowship from the American Association of University Women. This work is in partial fulfillment of the degree of Doctor of Philosophy for BAI.

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Israel, B.A., Schaeffer, W.I. Cytoplasmic mediation of malignancy. In Vitro Cell Dev Biol 24, 487–490 (1988). https://doi.org/10.1007/BF02628504

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Keywords

  • Parental Cell
  • Modal Number
  • Population Doubling
  • Malignant Phenotype
  • Early Passage