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Isolation and primary cultures of human intrahepatic bile ductular epithelium

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A technique for the isolation of human intrahepatic bile ductular epithelium, and the establishment of primary cultures using a serum- and growth-factor-supplemented medium combined with a connective tissue substrata is described. Initial cell isolates and monolayer cultures display phenotypic characteristics of biliary epithelial cells (low molecular weight prekeratin positive; albumin, alphafetoprotein, and Factor VIII-related antigen negative). Ultrastructural features of the cultured cells show cell polarization with surface microvilli, numerous interepithelial junctional complexes and cytoplasmic intermediate prekeratin filaments.

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Demetris, A.J., Markus, B.H., Saidman, S. et al. Isolation and primary cultures of human intrahepatic bile ductular epithelium. In Vitro Cell Dev Biol 24, 464–470 (1988).

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