Summary
The presence ofα 2-macroglobulin was detected with the avidin-biotin technique in more than 20-yr-old paraffin blocks from human sarcomas.α 2M was found mainly in the cytoplasm of the tumor cells, and almost all tumor cells were positive. This serum glycoprotein, which is a major plasma proteinase inhibitor with a wide specificity, was also shown to be synthesized and secreted by all three cell lines derived from primary sarcomas but was not detected in cultures of the autologous skin fibroblasts. For the detection ofα 2M in situ and in vitro an antiserum to tumor-associatedα 2-macroglobulin was used.
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Our work was supported by grant no. 55-B86-21XB, from the Swedish Cancer Society.
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Grófová, M., Larsson, E., Bengtsson, A. et al. Localization ofα 2-macroglobulin in human primary sarcomas and synthesis in established cell linesin human primary sarcomas and synthesis in established cell lines. In Vitro Cell Dev Biol 24, 369–372 (1988). https://doi.org/10.1007/BF02628487
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DOI: https://doi.org/10.1007/BF02628487