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Further cellular investigation of the human hepatoblastoma-derived cell line HepG2: Morphology and immunocytochemical studies of hepatic-secreted proteins

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The hepatoblastoma cell line HepG2 has been a matter of many investigations; most of them include biochemical studies of lipoprotein and other hepatic protein metabolism. However, the accurate cellular features of these cells have not been emphasized. We studied the cellular histologic, histochemical, and ultrastructural characteristics of this cell line. In addition, we investigated by immunoenzymatic methods the cellular biosynthesis of several proteins: apolipoproteins-AI,-B,-D, and-E, albumin, alpha-fetoprotein, transferrin, alpha-1-antitrypsin, C-reactive protein, fibronectin, and collagens I, III and IV. The rates of accumulation, in the medium of HepG2 cells, of albumin, alpha-1-antitrypsin, transferrin, and alpha-fetoprotein were 13.2±1.9; 4.9±1.5; 3.2±0.4; and 10.7±1.7 μg/106 cells/24 h, respectively. Our results show that HepG2 cells exhibited most cellular features of normal human hepatocytes. Bile canaliculi as well as Golgi apparatus complexes were particularly developed. Except for the C-reactive protein, HepG2 cells have all retained the ability to synthesize hepatic proteins but with some variable intensity from cell to cell. This hepatoblastoma cell line seems to represent a useful tool in the understanding of hepatic protein biosynthesis, particularly for the investigation on the secretory pathway of plasma proteins.

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Authors and Affiliations

  1. Laboratoire de Biologie Cellulaire, INSERM U24, Faculté de Médecine Xavier Bichat, 16 rue Henri Huchard, 75018, Paris, France

    Marie-Elisabeth Bouma, Edith Rogier, Nicole Verthier & Gérard Feldmann

  2. Laboratoire d'Immunologie, Hôpital Bichat, 75018, Paris, France

    Colette Labarre

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  1. Marie-Elisabeth Bouma
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  2. Edith Rogier
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  3. Nicole Verthier
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  4. Colette Labarre
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  5. Gérard Feldmann
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Bouma, ME., Rogier, E., Verthier, N. et al. Further cellular investigation of the human hepatoblastoma-derived cell line HepG2: Morphology and immunocytochemical studies of hepatic-secreted proteins. In Vitro Cell Dev Biol 25, 267–275 (1989). https://doi.org/10.1007/BF02628465

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  • DOI: https://doi.org/10.1007/BF02628465

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