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Distinction between vascular smooth muscle cells and myoepithelial cells in primary monolayer cultures of human breast tissue

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Summary

We report on the discrimination of vascular smooth muscle cells and myoepithelial cells in primary cultures of human breast tissue. Breast tissue was disaggregated enzymatically and the resulting organoids seeded in monolayer culture on collagen-coated plastic in serum-free medium CDM3a. Two main types of organoids were present after enzymatic digestion. One resembled small blood vessels and the other interlobular ducts or acini of the breast gland epithelium. Within 3 to 8 d after plating the organoids migrated into typical monolayer islets. These monolayer islets were evaluated using phase contrast microscopy and further tagged with monoclonal antibodies for immunocytochemical demonstration of Factor VIII-related antigen, muscle iso-forms of actin, type IV collagen, vimentin, desmin, and keratins. It is concluded that vascular smooth muscle cells resembled myoepithelial cells by expressing vimentin filaments, depositing type IV collagen, and showing immunoreactivity to muscle iso-forms of actin. However, whereas vascular smooth muscle cells were associated with endothelial cells and sometimes expressed desmin, myoepithelial cells appeared together with luminal epithelial cells and expressed cytokeratins.

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References

  1. Bartek, J.; Durban, E. M.; Hallowes, R. C., et al. A subclass of luminal epithelial cells in the human mammary gland, defined by antibodies to cytokeratins. J. Cell Sci. 75:17–33;1985.

    PubMed  CAS  Google Scholar 

  2. Brown, D. C.; Theaker, J. M.; Banks, P. M., et al. Cytokeratin expression in smooth muscle and smooth muscle tumours. Histopathology 11:477–486;1987.

    Article  PubMed  CAS  Google Scholar 

  3. Chamley-Campbell J. H.; Campbell, G. R.; Ross, R. Phenotype dependent response of cultured smooth muscle to serum mitogens. J. Cell Biol. 89:379–383;1981

    Article  PubMed  CAS  Google Scholar 

  4. Chen, J.-M.; Little, C. D. Cells that emerge from embryonic explants produce fibers of type IV collagen. J. Cell Biol. 101:1175–1181;1985.

    Article  PubMed  CAS  Google Scholar 

  5. Dairkee, S. H.; Blayney, C. M.; Asarnow, D. M., et al. Early expression of vimentin in human mammary cultures. In Vitro 24:321–327;1985.

    Google Scholar 

  6. Dairkee S. H.; Blayney-Moore, C. M.; Smith, H. S., et al. Concurrent expression of basal and luminal epithelial markers in cultures of normal human breast analysed using monoclonal antibodies. Differentiation 32:93–100;1986

    Article  PubMed  CAS  Google Scholar 

  7. Dropulic, B.; Masters, C. L. Culture of mouse brain capillary endothelial cell lines that express factor VII, ψ-glytamyl transpeptidase, and form junctional complexes in vitro. In Vitro 23:775–781;1987.

    CAS  Google Scholar 

  8. Franke, W. W.; Schmid, E.; Freudenstein, C., et al. Intermediate-sized filaments of the prekeratin type in myoepithelial cells. J. Cell Biol. 84:633–654;1980.

    Article  PubMed  CAS  Google Scholar 

  9. Garrels, J. I.; Gibson, W.. Identification and characterization of multiple forms of actin. Cell 9:793–805;1976.

    Article  PubMed  CAS  Google Scholar 

  10. Gown, A. M.; Boyd, H. C.; Chang, Y., et al. Smooth muscle cells can express cytokeratins of “simple” epithelium. Immunocytochemical and biochemical studies in vitro and in vivo. Am. J. Pathol. 132:223–232;1988.

    PubMed  CAS  Google Scholar 

  11. Gusterson, B. A.; Warburton, M. J.; Mitchell, D., et al. Invading squamous cell carcinoma can retain a basal lamina. Lab. Invest. 51:82–87;1984.

    PubMed  CAS  Google Scholar 

  12. Hoshi, H.; Kan, M.; Chan, J.-K., et al. Comparative endocrinology-paracrinology-autocrinology of human adult large vessel endothelia and smooth muscle cells. In Vitro 24:309–320;1988.

    CAS  Google Scholar 

  13. Jamieson, S.; Dunnington, D. J.; Ormerod, E. J., et al. Dedifferentiation of rat mammary myoepithelial-like cell lines after passage in vivo or cloning in vitro. J. Natl. Cancer Inst. 76:247–257;1986.

    PubMed  CAS  Google Scholar 

  14. Kocher, O.; Skalli, O.; Bloom, W. S., et al. Cytoskeleton of rat aortic smooth muscle cells. Lab. Invest. 50:645–651;1984.

    PubMed  CAS  Google Scholar 

  15. McGuire, P. G.; Orklin, R. W. Isolation of rat aortic endothelial cells by primary explant techniques and their phenotypic modulation by defined substrata. Lab. Invest. 57:94–105;1987.

    PubMed  CAS  Google Scholar 

  16. Moll, R.; Franke, W. W.; Schiller, D. L., et al. The catalogue of human cytokeratins: patterns of expression in normal epithelial tumors and cultured cells. Cell 31:11–24;1982.

    Article  PubMed  CAS  Google Scholar 

  17. Moyer, C. F.; Dennis, P. A.; Majno, G., et al. Venular endothelium in vitro: isolation and characterization. In Vitro 24:359–368;1986.

    Google Scholar 

  18. Osborn, M.; Debus, E.; Weber, K. Monoclonal antibodies specific for vimentin, Eur. J. Cell Biol. 34:137–143;1984.

    PubMed  CAS  Google Scholar 

  19. Owens, G. K.; Loeb, A.; Gordon, D., et al. Expression of smooth muscle-specific α-isoactin in cultured vascular smooth muscle cells: relationship, between growth and cytodifferentiation. J. Cell Biol. 102:343–352;1986.

    Article  PubMed  CAS  Google Scholar 

  20. Petersen, O. W.; Hoyer, P. E.; Hilgers, J., et al. Characterization of epithelial cell islets in primary monolayer cultures of human breast carcinomas by the tetrazolium reaction for glucose 6-phosphate dehydrogenase. Virch. Arch. (Cell Pathol.) 50:27–42;1985.

    Article  CAS  Google Scholar 

  21. Petersen, O. W.; van Deurs, B. Demonstration of human breast carcinoma cells in cryosections and primary monolayer cultures of surgical biopsies by neotetrazolium reductase cytochemistry. Cancer Res. 46:2013–2020;1986

    PubMed  CAS  Google Scholar 

  22. Petersen, O. W.; van Deurs, B. Preservation of defined phenotypic traits in short-term cultured human breast carcinoma derived epithelial cells. Cancer Res. 47:856–866;1987.

    PubMed  CAS  Google Scholar 

  23. Rudland, P. S. Stem cells and the development of mammary cancers in experimental rats and humans. Cancer Metastasis Rev. 6:55–83;1987.

    Article  PubMed  CAS  Google Scholar 

  24. Skalli, O.; Ropraz, P.; Trseciak, A. et al. A monoclonal antibody against α-smooth muscle actin: a new probe for smooth muscle differentiation. J. Cell Biol. 103:2787–2796;1986.

    Article  PubMed  CAS  Google Scholar 

  25. Taylor-Papadimitriou, J.; Lane, E. B.; Chang, S. E. Cell lineages and interactions in neoplastic expression in the human breast. In: Rich, M. A.; Hager, J. C.; Furmanski, P., eds. Understanding breast cancer, clinical and laboratory concepts. New York, Basel: Marcel Dekker; 1984:215–246.

    Google Scholar 

  26. Thyberg, J.; Palmberg, L.; Nilsson, J., et al. Phenotype modulation in primary cultures of arterial smooth muscle cells. Differentiation 25:156–167;1983.

    Article  PubMed  CAS  Google Scholar 

  27. Tsukada, T.; Tippens, D.; Gordon, D., et al. HHF-35, a muscleactin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization. Am. J. Pathol. 126:51–60; 1987.

    PubMed  CAS  Google Scholar 

  28. Turley, H.; Pulford, K. A. F.; Gatter, K. C., et al. Biochemical evidence that cytokeratins are present in smooth muscle. J. Exp. Pathol. 69:433–440;1988

    CAS  Google Scholar 

  29. Wagner, D. D.; Olmsted, J. B.; Marder, V. J. Immunolocalization of von Willebrand protein in Weibel-Palade bodies of human endothelial cells. J. Cell Biol. 95:355–360; 1982.

    Article  PubMed  CAS  Google Scholar 

  30. Warburton, M. J.; Ferns, S. A.; Hughes, C. M., et al. Generation of cell types with myoepithelial and mesenchymal phenotypes during the conversion of rat mammary tumor epithelial stem cells into elongated cells. JNCI 78:1191–1201;1987.

    PubMed  CAS  Google Scholar 

  31. Yang, J.; Balakrishnan A.; Hamamoto, S., et al. Different mitogenic and phenotypic responses of human breast epithelial cells grown in two versus three dimensions. Exp. Cell Res. 167:563–569;1986.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Structural Cell Biology Unit, Department of Anatomy, The Panum Institute, DK-2200, Copenhagen N, Denmark

    O. W. Petersen & B. Van Deurs

  2. Laboratory of Tumor Endocrinology, The Fibiger Institute, DK-2100, Copenhagen O, Denmark

    O. W. Petersen

Authors
  1. O. W. Petersen
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  2. B. Van Deurs
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This work was supported by the Danish Medical Research Council, the Danish Cancer Society, the NOVO Foundation, and the Thaysen Foundation.

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Petersen, O.W., Van Deurs, B. Distinction between vascular smooth muscle cells and myoepithelial cells in primary monolayer cultures of human breast tissue. In Vitro Cell Dev Biol 25, 259–266 (1989). https://doi.org/10.1007/BF02628464

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  • DOI: https://doi.org/10.1007/BF02628464

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