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Identification of newly synthesized wing imaginal disc proteins induced by 20-hydroxyecdysone inGalleria mellonella

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Summary

Wing imaginal discs from 7th instarGalleria mellonella L. larvae evaginate and exhibit tracheolar elongation when exposed to 20-hydroxyecdysone in vitro. This response was elicited within 24 h of treatment as was a greater than fourfold stimulation of the incorporation of [3H]leucine into disc proteins. Autoradiographic analyses of [35S]methionine labeled polypeptides separated on two-dimensional gels, however, revealed no differences in protein profiles between control and treated discs until 48 h following exposure to molting hormone. At this time, wing imaginal discs exposed to 1 μg/ml 20-hydroxyecdysone synthesized four unique polypeptides not detected either in controls or in discs treated for 24 h. These four new proteins were also found to be synthesized by imaginal discs that had evaginated in vivo. These results suggest that these proteins are normally synthesized subsequent to evagination and do not play a role in the morphological events necessary for evagination.

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Authors and Affiliations

  1. Department of Entomology and Nematology, University of Florida, P.O. Box 14565, 32604, Gainesville, FL

    S. G. Miller

  2. Insect Attractants, Behavior and Basic Biology Research Laboratory, Agricultural Research, Science and Education Administration, USDA, P.O. Box 14565, 32604, Gainesville, FL

    H. Oberlander

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  1. S. G. Miller
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  2. H. Oberlander
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Mention of a commercial or proprietary product in this paper does not constitute an endorsement of that product by the USDA. S. G. M. is employed through a cooperative agreement between the Insect Attractants, Behavior and Basic Biology Laboratory and the Department of Entomology, University of Florida.

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Miller, S.G., Oberlander, H. Identification of newly synthesized wing imaginal disc proteins induced by 20-hydroxyecdysone inGalleria mellonella . In Vitro 17, 689–694 (1981). https://doi.org/10.1007/BF02628404

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  • DOI: https://doi.org/10.1007/BF02628404

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