Skip to main content
SpringerLink
Log in

Yield and activity of theHeliothis zea single nuclear polyhedrosis virus propagated in cloned and uncloned lines ofHeliothis cells

  • Plant Cellular and Developmental Biology
  • Published:
In Vitro Cellular & Developmental Biology Aims and scope Submit manuscript

Summary

Heliothis cell lines originated from different laboratories were characterized by isoenzyme analysis and then evaluated for their ability to produce the single nuclear polyhedrosis virus ofHeliothis zea (HzSNPV). A cloned cell line (designated Hzlb3), whose homogeneity was supported by both morphological and isoenzyme analysis, was derived from a parental line (Hzl). Significantly greater yields (about 10-fold) of tissue-culture-derived, non-occluded virus (TCNOV) were obtained when compared to the parental line. The Hzlb3 clone also gave significantly higher yields of TCNOV than the Hz3 and UND-K cell lines. Although lines Hzl, Hz3, and Hzlb3 produced significantly more polyhedral inclusion bodies (PIB) than line UND-K, the infectivity of PIB from UND-K equaled that of lines Hzl and Hzlb3.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Brown, S. E.; Knudson, D. L. Characterization of invertebrate cell lines. III Isozyme analyses employing cellulose-acetate electrophoresis. In Vitro 16:829–832; 1980.

    Article  CAS  Google Scholar 

  2. Corsaro, B. G.; Fraser, M. J. Characterization of clonal populations of theHeliothis zea cell line IPLB-Hz1075. In Vitro Cell. Dev. Bio. 23:855–862; 1987.

    Article  Google Scholar 

  3. Gettig, R. R.; Burand, J. P.; Flore, P. H.; McCarthy, W. J.; Wood, H. A. Replication ofHeliothis zea nuclear polyhedrosis virus in insect cell culture: virus-induced and structural proteins. J. Gen. Virol. 68:1617–1625; 1987.

    CAS  Google Scholar 

  4. Goodwin, R. H.; Vaughn, J. L.; Adams, J. R.; Louloudes, S. J. Replication of a nuclear polyhedrosis virus in an established insect cell line. J. Invertebr. Pathol. 16:284–288; 1970.

    Article  PubMed  CAS  Google Scholar 

  5. Goodwin, R. H.; Topkins, G. J.; Gettig, R. R.; Adams, J. R. Characterization and culture of virus replicating continuous insect cell lines from the bollworm,Heliothis zea (Boddie). In Vitro 18:843–850; 1982.

    Article  Google Scholar 

  6. Granados, R. R.; Lawler, K. A.; Burand, J. P. Replication ofHeliothis zea baculovirus in an insect cell line. Intervirology 16:71–79; 1981.

    PubMed  CAS  Google Scholar 

  7. Hink, W. F.; Vail, P. V. A plaque assay for titration of alfalfa looper nuclear polyhedrosis virus in a cabbage looper (TN-368) cell line. J. Invertebr. Pathol. 22:168–174; 1973.

    Article  Google Scholar 

  8. Ignoffo, C. M.; McIntosh, A. H.; Garcia, C. Susceptibility of larvae ofHeliothis zea, H. virescens, andH. armigera (Lep.: Noctuidae) to three baculoviruses. Entomophaga 28:1–8; 1983.

    Article  Google Scholar 

  9. McIntosh, A. H.; Ignoffo, C. M.; Andrews, P.In vitro host range of five baculoviruses in lepidopteran cell lines. Intervirology 23:150–156; 1985.

    PubMed  CAS  Google Scholar 

  10. McIntosh, A. H.; Ignoffo, C. M. Replication and infectivity of the single-embedded nuclear polyhedrosis virus, Baculovirus heliothis, in homologous cell lines. J. Invertebr. Pathol. 37:258–264; 1981.

    Article  Google Scholar 

  11. McIntosh, A. H.; Ignoffo, C. M. Characterization of five cell lines established from species ofHeliothis. Appl. Entomol. Zool. 18:262–269; 1983.

    Google Scholar 

  12. McIntosh, A. H.; Maramorosch, K.; Rechtoris, C. Adaptation of an insect cell line (Agallia constricta) in a mammalian cell culture medium. In Vitro 8:375–378; 1973.

    Article  PubMed  CAS  Google Scholar 

  13. McIntosh, A. H.; Rechtoris, C. Insect cells: colony formation and cloning in agar medium. In Vitro 10:1–5; 1974.

    Article  PubMed  CAS  Google Scholar 

  14. Sohi, S. S.; Percy, J.; Arif, B. M.; Cunningham, J. C. Replication and serial passage of singly enveloped baculovirus ofOrygia leucostigma in homologous cell lines. Intervirology 21:50–60; 1984.

    Article  PubMed  CAS  Google Scholar 

  15. Wood, H. A.; Johnston, L. B.; Buraud, J. P. Inhibition ofAutographa californica nuclear polyhedrosis virus replication in high densityTrichoplusia ni cell cultures. Virology 119:245–254; 1982.

    Article  CAS  PubMed  Google Scholar 

  16. Yamada, K-I.; Sherman, K. E.; Maramorosch, K. Serial passage ofHeliothis zea singly embedded nuclear polyhedrosis virus in a homologous cell line. J. Invertebr. Pathol. 39:185–191; 1982.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biological Control of Insects Research Laboratory, Agricultural Research Service, U. S. Department of Agriculture, P. O. Box 7629, 65205, Columbia, Research Park, Missouri

    W. C. Rice, A. H. McIntosh & C. M. Ignoffo

Authors
  1. W. C. Rice
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. H. McIntosh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. M. Ignoffo
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rice, W.C., McIntosh, A.H. & Ignoffo, C.M. Yield and activity of theHeliothis zea single nuclear polyhedrosis virus propagated in cloned and uncloned lines ofHeliothis cells. In Vitro Cell Dev Biol 25, 201–204 (1989). https://doi.org/10.1007/BF02626179

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02626179

Key words

Search

Navigation